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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab54868? Please let us know so that we can cite the reference in this datasheet
ab54868 has been referenced in 2 publications.
- Custódio MR et al. Parathyroid hormone and phosphorus overload in uremia: impact on cardiovascular system. Nephrol Dial Transplant : (2011). PubMed: 21825304
- Graciolli FG et al. Phosphorus overload and PTH induce aortic expression of Runx2 in experimental uraemia. Nephrol Dial Transplant 24:1416-21 (2009). PubMed: 19075196
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - RUNX2 antibody (ab54868)
All lanes : Anti-RUNX2 antibody (ab54868) at 1/1000 dilution
Lane 1 : UMR-106 Rat osteosarcoma cell line, nuclear cell lysate
Lane 2 : UMR-106 Rat osteosarcoma cell line, cytoplasmic cell lysate
Secondary
HRP conjugated goat anti-mouse antibody at 1/500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57/59,65,98 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
The blot was blocked with 1% milk for 1 hour at RT and incubated with the primary antibody for 1 hour.
This image is courtesy of an Abreview submitted by Dr Cesar Camacho
Western blot - RUNX2 antibody (ab54868)
Anti-RUNX2 antibody (ab54868) at 1 µg/ml + Cell lysate prepared from K-562 cells
Predicted band size : 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX2 antibody (ab54868)
ab54868 at 3 ug/ml staining RUNX2 in human placenta tissue section by Immunohistochemistry (Formalin /PFA fixed paraffin embedded tissue sections).
RUNX2 antibody for WB in Rat (54868)
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