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We purchased ab54868, mouse monoclonal antibody against runx2. We seem to have a problem with it however. The antibody is staining beautifully, but it is not staining the targets that it should. Our immunohistochemistry patterning is showing muscle while this is a bone marker, and it is not showing up in bones that are known to contain runx2. Our western blotting is giving bands at 170 and 200kDa while the known size of our zebrafish runx2 is 53 kDa. We would prefer a replacement over a refund if that is possible. |
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Thank you for contacting Abcam regarding ab54868. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-RUNX2 antibody (ab54868) at 1/1000 dilution
Lane 1 : UMR-106 Rat osteosarcoma cell line, nuclear cell lysate
Lane 2 : UMR-106 Rat osteosarcoma cell line, cytoplasmic cell lysate
Secondary
HRP conjugated goat anti-mouse antibody at 1/500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57/59,65,98 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
The blot was blocked with 1% milk for 1 hour at RT and incubated with the primary antibody for 1 hour.
This image is courtesy of an Abreview submitted by Dr Cesar Camacho
Anti-RUNX2 antibody (ab54868) at 1 µg/ml + Cell lysate prepared from K-562 cells
Predicted band size : 57 kDa
ab54868 at 3 ug/ml staining RUNX2 in human placenta tissue section by Immunohistochemistry (Formalin /PFA fixed paraffin embedded tissue sections).
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