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Anti-RUNX2 antibody (ab54868)

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Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab54868 for help.

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1 question for ab54868

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Question 1

Monday 30-January-2012

We purchased ab54868, mouse monoclonal antibody against runx2. We seem to have a problem with it however. The antibody is staining beautifully, but it is not staining the targets that it should. Our immunohistochemistry patterning is showing muscle while this is a bone marker, and it is not showing up in bones that are known to contain runx2. Our western blotting is giving bands at 170 and 200kDa while the known size of our zebrafish runx2 is 53 kDa. We would prefer a replacement over a refund if that is possible.

ANSWER:

 

Thank you for contacting Abcam regarding ab54868.

I am sorry that you have been experiencing difficulties with this antibody in WB and IHC. I would like to get some additional information regarding the samples used and the conditions you are testing. Would you please provide a detailed summary of the protocols you have tested for WB and IHC? Sample species and preparation would also be helpful. If we find that the antibody is not working as stated on the datasheet, I would be happy to offer a replacement. Please also provide your order number so that I can locate it in our system and confirm that you are contacting us within 6 months of purchase, per our Abpromise guarantee.

I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions or concerns.

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