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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab81357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionTranscription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.
  • Tissue specificitySpecifically expressed in osteoblasts.
  • Involvement in diseaseDefects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.
  • Sequence similaritiesContains 1 Runt domain.
  • DomainA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.
  • Post-translational
    modifications    Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q13950 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Acute myeloid leukemia 3 protein antibodyAlpha subunit 1 antibodyAML3 antibody
      CBF alpha 1 antibodyCBF-alpha-1 antibodyCBFA1 antibodyCCD antibodyCCD1 antibodyCleidocranial dysplasia 1 antibodyCore binding factor antibodyCore binding factor runt domain alpha subunit 1 antibodyCore binding factor subunit alpha 1 antibodyCore-binding factor subunit alpha-1 antibodyMGC120022 antibodyMGC120023 antibodyOncogene AML 3 antibodyOncogene AML-3 antibodyOSF 2 antibodyOSF-2 antibodyOSF2 antibodyOsteoblast specific transcription factor 2 antibodyOsteoblast-specific transcription factor 2 antibodyOTTHUMP00000016533 antibodyPEA2 alpha A antibodyPEA2-alpha A antibodyPEA2aA antibodyPEBP2 alpha A antibodyPEBP2-alpha A antibodyPEBP2A1 antibodyPEBP2A2 antibodyPEBP2aA antibodyPEBP2aA antibodyPEBP2aA1 antibodyPolyomavirus enhancer binding protein 2 alpha A subunit antibodyPolyomavirus enhancer-binding protein 2 alpha A subunit antibodyRunt domain antibodyRunt related transcription factor 2 antibodyRunt-related transcription factor 2 antibodyRUNX2 antibodyRUNX2_HUMAN antibodySL3 3 enhancer factor 1 alpha A subunit antibodySL3-3 enhancer factor 1 alpha A subunit antibodySL3/AKV core binding factor alpha A subunit antibodySL3/AKV core-binding factor alpha A subunit antibody
    see all

Anti-RUNX2 antibody images

  • Western blot - RUNX2 antibody (ab81357)
    Western blot - RUNX2 antibody (ab81357)
    All lanes : Anti-RUNX2 antibody (ab81357) at 1 µg/ml

    Lane 1 : Tissue lysates prepared from Rat thymus tissue.
    Lane 2 : Tissue lysates prepared from Rat testicular tissue.

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 56 kDa
  • Western blot - RUNX2 antibody (ab81357)
    Western blot - RUNX2 antibody (ab81357)
    All lanes : Anti-RUNX2 antibody (ab81357) at 1 µg/ml

    Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-RUNX2 antibody (ab81357)

ab81357 has not yet been referenced specifically in any publications.

Publishing research using ab81357 ? Please let us know so that we can cite the reference in this datasheet

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