Anti-RUNX3 antibody [2B3] (ab135248)
Key features and details
- Mouse monoclonal [2B3] to RUNX3
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-RUNX3 antibody [2B3]
See all RUNX3 primary antibodies -
Description
Mouse monoclonal [2B3] to RUNX3 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 186-252 of Human RUNX3 expressed in E. Coli.
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Positive control
- Human RUNX3 recombinant protein; HEK293 cell lysate transfected with RUNX3 (aa186-252)-hIgGFc; NIH 3T3 cells; Human cervical cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR206339-20 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2B3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 44 kDa.
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IHC-P | (1) |
1/200 - 1/1000.
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Flow Cyt | (1) |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 44 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. -
Sequence similarities
Contains 1 Runt domain. -
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes. -
Post-translational
modificationsPhosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. -
Cellular localization
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 864 Human
- Entrez Gene: 12399 Mouse
- Omim: 600210 Human
- SwissProt: Q13761 Human
- SwissProt: Q64131 Mouse
- Unigene: 170019 Human
- Unigene: 378894 Mouse
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Alternative names
- Acute myeloid leukemia 2 protein antibody
- Acute myeloid leukemia gene 2 antibody
- AML 2 antibody
see all
Images
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Immunohistochemical analysis of Paraffin-embedded Human cervical cancer tissue labelling RUNX3 with ab135248 at 1/200 dilution followed by DAB staining.
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: RUNX3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab135248 observed at 44 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab135248 was shown to recognize RUNX3 in wild-type HAP1 cells as signal was lost at the expected MW in RUNX3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RUNX3 knockout samples were subjected to SDS-PAGE. Ab135248 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
Flow cytometric analysis of NIH 3T3 cells labelling RUNX3 with ab135248 at 1/200 dilution (green). Negative control (red).
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Anti-RUNX3 antibody [2B3] (ab135248) at 1/500 dilution + Recombinant Human RUNX3 protein
Predicted band size: 44 kDa -
All lanes : Anti-RUNX3 antibody [2B3] (ab135248) at 1/500 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with RUNX3 (amino acids 186-252)-hIgGFc
Predicted band size: 44 kDa
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab135248 has been referenced in 13 publications.
- Chen X et al. RUNX3/H3K27me3 Co-Expression Defines a Better Prognosis in Surgically Resected Stage I and Postoperative Chemotherapy-Naive Non-Small-Cell Lung Cancer. J Oncol 2022:5752263 (2022). PubMed: 35368900
- Nagata K et al. Runx2 and Runx3 differentially regulate articular chondrocytes during surgically induced osteoarthritis development. Nat Commun 13:6187 (2022). PubMed: 36261443
- Yang RN et al. Antitumor activity of RUNX3: Upregulation of E-cadherin and downregulation of the epithelial-mesenchymal transition in clear-cell renal cell carcinoma. Open Life Sci 17:1579-1590 (2022). PubMed: 36518886
- Thomsen I et al. RUNX1 Regulates a Transcription Program That Affects the Dynamics of Cell Cycle Entry of Naive Resting B Cells. J Immunol 207:2976-2991 (2021). PubMed: 34810221
- Pang L et al. Fine particulate matter induces airway inflammation by disturbing the balance between Th1/Th2 and regulation of GATA3 and Runx3 expression in BALB/c mice. Mol Med Rep 23:N/A (2021). PubMed: 33760131