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ab40278 has been referenced in 6 publications.
Publishing research using ab40278? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 44 kDa
Additional bands at : 46 kDa (possible isoform).
IHC image of RUNX3 staining in human lymphoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40278, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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