![Western blot - RUNX3 antibody [R3-5G4] (ab40278) image](#ab40278_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278) image](#ab40278-ap538130.jpg)
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Overview
Product name
Anti-RUNX3 antibody [R3-5G4]
See all RUNX3 products (7) ...
Description
Mouse monoclonal [R3-5G4] to RUNX3
Tested applications
IP, WB, EMSA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment derived from residues 191 - 300 of Human RUNX3.
Positive control
ab40278 gave a positive result in the following whole cell lysates: Jurkat (Human T cell lymphoblast-like cell line) Raji (Human Burkitt's lymphoma cell line) SW480 (Human colon adenocarcinoma cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
R3-5G4
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
- 1 Image
WB
EMSA
Application notes
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
EMSA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational
modifications
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Cellular localization
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
Target information above from: UniProt accessionQ13761
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acute myeloid leukemia 2 protein antibody
- Acute myeloid leukemia gene 2 antibody
- AML 2 antibody
see all
Database links
- Entrez Gene: 864 Human
- Omim: 600210 Human
- SwissProt: Q13761 Human
- Unigene: 170019 Human
Anti-RUNX3 antibody [R3-5G4] images:
Western blot - RUNX3 antibody [R3-5G4] (ab40278)
![Western blot - RUNX3 antibody [R3-5G4] (ab40278)](/ps/datasheet/Images/40/ab40278/ab40278_1.jpg)
All lanes : Anti-RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 44 kDa
Additional bands at : 46 kDa (possible isoform).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278)](/ps/datasheet/Images/40/ab40278/ab40278-ap538130.jpg)
IHC image of RUNX3 staining in human lymphoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40278, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-RUNX3 antibody [R3-5G4] (ab40278)
This product has been referenced in:
- Yano Tet al. The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis. Mol Cell Biol 26:4474-88 (2006).Read more (PubMed: 16738314) »
- Fu Yet al. RUNX3 Maintains the Mesenchymal Phenotype after Termination of the Notch Signal. J Biol Chem 286:11803-13 (2011). WB; Human.Read more (PubMed: 21288908) »
See all 6 publications for this product
Publishing research using ab40278? Please let us know so that we can cite the reference in this datasheet
![RUNX3 antibody [R3-5G4] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (40278)](/ps/datasheet/images/40/ab40278/RUNX3-Primary-antibodies-ab40278-4.jpg)
![RUNX3 antibody [R3-5G4] for ChIP in Human (40278)](/ps/datasheet/images/40/ab40278/RUNX3-Primary-antibodies-ab40278-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"