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Western blot - RUNX3 antibody [R3-5G4] (ab40278)
![Western blot - RUNX3 antibody [R3-5G4] (ab40278)](/ps/datasheet/images/ab40278_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278)](/ps/datasheet/images/ab40278-ap538130.jpg)
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Product Name
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RUNX3 antibody [R3-5G4]
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See all RUNX3 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [R3-5G4] to RUNX3
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Immunogen
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Recombinant fragment derived from residues 191 - 300 of Human RUNX3.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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EMSA, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
EMSA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab40278 gave a positive result in the following whole cell lysates:
Jurkat (Human T cell lymphoblast-like cell line)
Raji (Human Burkitt's lymphoma cell line)
SW480 (Human colon adenocarcinoma cell line)
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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R3-5G4
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RUNX3 antibody [R3-5G4] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RUNX3 antibody [R3-5G4] images, references, products related to ab40278 and other tools.
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RUNX3 antibody [R3-5G4] images:
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 Western blot - RUNX3 antibody [R3-5G4] (ab40278)
All lanes : RUNX3 antibody [R3-5G4] (ab40278) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Rabbit Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 44 kDa
Additional bands at : 46 kDa (possible isoform).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278)
IHC image of RUNX3 staining in human lymphoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40278, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for RUNX3 antibody [R3-5G4] (ab40278)
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This product has been used in: (two most recent references)
Sakakura C et al. Frequent silencing of RUNX3 in esophageal squamous cell carcinomas is associated with radioresistance and poor prognosis. Oncogene 26:5927-38 (2007). PubMed: 17384682
Salto-Tellez M et al. RUNX3 protein is overexpressed in human basal cell carcinomas. Oncogene 25:7646-9 (2006). PubMed: 16767156
See all 4 publication references for this product.
If you publish research using ab40278 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RUNX3
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RUNX3 antibody [R3-5G4] - more information
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Customer reviews (feedback) regarding RUNX3 antibody [R3-5G4] |
Customer FAQs regarding RUNX3 antibody [R3-5G4] |
Protocols for RUNX3 antibody [R3-5G4] |
Price and availability of products related to RUNX3 antibody [R3-5G4] |
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RUNX3 antibody [R3-5G4] - related products:
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RUNX3 antibody [R3-5G4] - other tools:
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Contact Abcam with a Technical Enquiry about RUNX3 antibody [R3-5G4] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - RUNX3 antibody [R3-5G4] (ab40278)](/index.html?datasheet=40278#ab40278_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX3 antibody [R3-5G4] (ab40278)](/index.html?datasheet=40278#ab40278-ap538130.jpg)
