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Electrophoretic Mobility Shift Assay - RUNX3 antibody (ab11905)
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Product Name
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RUNX3 antibody
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See all RUNX3 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RUNX3
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
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Hu
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Tested applications
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EMSA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
EMSA: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji nuclear extract.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide and 0.01% thimerosal
Constituents: PBS, 0.2% gelatin Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RUNX3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RUNX3 antibody images, references, products related to ab11905 and other tools.
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RUNX3 antibody images:
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 Electrophoretic Mobility Shift Assay - RUNX3 antibody (ab11905)
Detection of AML2 by EMSA analysis. Nushift polyclonal antibody against AML2 factor creates a supershift in EMSA (lane 4).
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References for RUNX3 antibody (ab11905)
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This product has been used in: (two most recent references)
Chen PC et al. Novel roles for MLH3 deficiency and TLE6-like amplification in DNA mismatch repair-deficient gastrointestinal tumorigenesis and progression. PLoS Genet 4:e1000092 (2008). WB. PubMed: 18551179
Cheng CK et al. Transcriptional repression of the RUNX3/AML2 gene by the t(8;21) and inv(16) fusion proteins in acute myeloid leukemia. Blood : (2008). PubMed: 18663147
If you publish research using ab11905 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RUNX3
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RUNX3 antibody - more information
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Customer reviews (feedback) regarding RUNX3 antibody |
Customer FAQs regarding RUNX3 antibody |
Protocols for RUNX3 antibody |
Price and availability of products related to RUNX3 antibody |
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RUNX3 antibody - related products:
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RUNX3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RUNX3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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