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Overview
Product name
Anti-RUNX3 antibody - ChIP Grade
See all RUNX3 products (7) ...
Description
Rabbit polyclonal to RUNX3 - ChIP Grade
Tested applications
ChIP, WB, EMSAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Positive control
Raji nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide and 0.01% thimerosal
Constituents: PBS, 0.2% gelatin
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
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Electrophoretic Mobility Shift Assay - RUNX3 antibody (ab11905)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 20714105)
WB: 1/500 - 1/1000.Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
EMSA: Use at an assay dependent dilution.
Target
Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational
modifications
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Cellular localization
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
Target information above from: UniProt accessionQ13761
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acute myeloid leukemia 2 protein antibody
- Acute myeloid leukemia gene 2 antibody
- AML 2 antibody
see all
Database links
- Entrez Gene: 864 Human
- Omim: 600210 Human
- SwissProt: Q13761 Human
- Unigene: 170019 Human
Anti-RUNX3 antibody - ChIP Grade images:
Electrophoretic Mobility Shift Assay - RUNX3 antibody (ab11905)
Detection of AML2 by EMSA analysis. Nushift polyclonal antibody against AML2 factor creates a supershift in EMSA (lane 4).
References for Anti-RUNX3 antibody - ChIP Grade (ab11905)
This product has been referenced in:
- Ciavatta DJet al. Epigenetic basis for aberrant upregulation of autoantigen genes in humans with ANCA vasculitis. J Clin Invest 120:3209-19 (2010). WB, ChIP; Human.Read more (PubMed: 20714105) »
- Weersma RKet al. Runt-related transcription factor 3 is associated with ulcerative colitis and shows epistasis with solute carrier family 22, members 4 and 5. Inflamm Bowel Dis 14:1615-22 (2008). IHC-P; Human.Read more (PubMed: 18668679) »
See all 4 publications for this product
Publishing research using ab11905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"