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Overview
Product name
Anti-RUNX3 antibody
See all RUNX3 products (7) ...
Description
Rabbit polyclonal to RUNX3
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
A region within synthetic peptide: GAPQSQSGPF QANPAPYHLF YGASSGSYQF SMAAAGGGER SPTRMLTSCP, corresponding to C terminal amino acids 326-375 of Mouse RUNX3
GAPQSQSGPF QANPAPYHLF YGASSGSYQF SMAAAGGGER SPTRMLTSCP
Positive control
NIH3T3 cell lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - RUNX3 antibody (ab49117)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/12500ELISA: 1/12500
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P: Use at an assay dependent dilution. (PubMed: 21205319)
Target
Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational
modifications
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Cellular localization
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
Target information above from: UniProt accessionQ13761
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acute myeloid leukemia 2 protein antibody
- Acute myeloid leukemia gene 2 antibody
- AML 2 antibody
see all
Database links
- Entrez Gene: 864 Human
- Entrez Gene: 12399 Mouse
- Entrez Gene: 156726 Rat
- Omim: 600210 Human
- SwissProt: Q13761 Human
- SwissProt: Q64131 Mouse
- SwissProt: Q91ZK1 Rat
- Unigene: 170019 Human
see all
Anti-RUNX3 antibody images:
Western blot - RUNX3 antibody (ab49117)
Anti-RUNX3 antibody (ab49117) at 1.25 µg/ml + NIH3T3 cell lysate at 10 µg
Predicted band size : 44 kDa
Observed band size : 44 kDa
References for Anti-RUNX3 antibody (ab49117)
This product has been referenced in:
- Nakanishi Yet al. Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis. BMC Cancer 11:3 (2011). WB, IHC-P; Human.Read more (PubMed: 21205319) »
- Sheng Zet al. Transcription and signalling pathways involved in BCR-ABL-mediated misregulation of 24p3 and 24p3R. EMBO J 28:866-76 (2009). WB; Mouse.Read more (PubMed: 19229297) »
See all 2 publications for this product
Publishing research using ab49117? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"