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Immunofluorescence on mouse cerebellum (4% PFA fixed, frozen section) at 1/500 dilution using rabbit antibody to rat, mouse Runt-related transcription factor 3 (Runx3): whole serum. DAPI counterstain appearing in blue.
ab68938 (1/2000) staining RUNX3 in 1-day-old mouse corticla cell culture by immunocytochemistry/immunofluoresence (ICC/IF).
Anti-RUNX3 antibody (ab68938) at 1/1000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 31 kDa,52 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
1
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