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Overview
Product name
Anti-RUNX3 antibody
See all RUNX3 products (7) ...
Description
Rabbit polyclonal to RUNX3
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
A synthetic peptide as a part of rat and mouse RUNX3 conjugated to an immunogenic carrier protein
Properties
Form
Lyophilised:
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Concentration
Concentration information loading...
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/3000.Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P: 1/300 - 1/3000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/300 - 1/3000.
Target
Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational
modifications
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Cellular localization
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
Target information above from: UniProt accessionQ13761
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acute myeloid leukemia 2 protein antibody
- Acute myeloid leukemia gene 2 antibody
- AML 2 antibody
see all
Database links
- Entrez Gene: 12399 Mouse
- Entrez Gene: 156726 Rat
- SwissProt: Q64131 Mouse
- SwissProt: Q91ZK1 Rat
- Unigene: 378894 Mouse
Anti-RUNX3 antibody images:
Immunohistochemistry (Frozen sections) - RUNX3 antibody (ab68938)
Immunofluorescence on mouse cerebellum (4% PFA fixed, frozen section) at 1/500 dilution using rabbit antibody to rat, mouse Runt-related transcription factor 3 (Runx3): whole serum. DAPI counterstain appearing in blue.
Immunocytochemistry/ Immunofluorescence - RUNX3 antibody (ab68938)
ab68938 (1/2000) staining RUNX3 in 1-day-old mouse corticla cell culture by immunocytochemistry/immunofluoresence (ICC/IF).
Western blot - RUNX3 antibody (ab68938)
Anti-RUNX3 antibody (ab68938) at 1/1000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 31 kDa,52 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-RUNX3 antibody (ab68938)
ab68938 has not yet been referenced specifically in any publications.
Publishing research using ab68938? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"