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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-RYK antibody [F30 P7 D7 F5]
See all RYK products (4) ...
Description
Mouse monoclonal [F30 P7 D7 F5] to RYK
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Xenopus laevis
Immunogen
Synthetic peptide: CLTEFHAALGAYV conjugated to Ovalbumin, corresponding to amino acids 462 - 475 of RYK.
CLTEFHAALG AYV
Positive control
HCT116 cells lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
F30 P7 D7 F5
Isotype
IgG1
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlPredicted molecular weight: 67 kDa.
Target
Function
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Tissue specificity
Observed in all the tissues examined.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 protein kinase domain.
Contains 1 WIF domain.
Domain
The extracellular WIF domain is responsible for Wnt binding.
Post-translational
modifications
Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation.
Cellular localization
Membrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
Target information above from: UniProt accessionP34925
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D3S3195 antibody
- ERK 3 antibody
- Hydroxyaryl protein kinase antibody
see all
Database links
- Entrez Gene: 6259 Human
- Entrez Gene: 20187 Mouse
- Entrez Gene: 140585 Rat
- Omim: 600524 Human
- SwissProt: P34925 Human
- SwissProt: Q01887 Mouse
- SwissProt: Q8K272 Mouse
- SwissProt: Q6BC88 Rat
see all
References for Anti-RYK antibody [F30 P7 D7 F5] (ab33519)
ab33519 has not yet been referenced specifically in any publications.
Publishing research using ab33519? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"