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- Secondary antibodies
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Product name
Anti-RYK antibody
See all RYK products (5) ...
Description
Rabbit polyclonal to RYK
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 576-592 of Human RYK.
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 67.5 kDa (predicted molecular weight: 72 kDa).
Target
Function
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Tissue specificity
Observed in all the tissues examined.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 protein kinase domain.
Contains 1 WIF domain.
Domain
The extracellular WIF domain is responsible for Wnt binding.
Post-translational
modifications
Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation.
Cellular localization
Membrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
Target information above from: UniProt accessionP34925
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D3S3195 antibody
- ERK 3 antibody
- Hydroxyaryl protein kinase antibody
see all
Database links
- Entrez Gene: 6259 Human
- Omim: 600524 Human
- SwissProt: P34925 Human
- Unigene: 245869 Human
- Unigene: 654562 Human
Anti-RYK antibody images:
Western blot - RYK antibody (ab5518)
Predicted band size : 72 kDa
ab5518 at a 1/100 dilution staining approximately 67.5kDa band of RYK in Jurkat cell lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RYK antibody (ab5518)
ab5518 staining RYK in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-RYK antibody (ab5518)
ab5518 has not yet been referenced specifically in any publications.
Publishing research using ab5518? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"