|
|
|
|
Western blot - Rab3A antibody (ab3335)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - Rab3A antibody (ab3335)
(enlarge)
|
|
|
|
|
Product Name
|
Rab3A antibody
|
| |
See all Rab3A antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Rab3A
|
|
Immunogen
|
Synthetic Peptide: M(1)ASATDS RYGQKESSDQ N(18)C.
Immunizin peptide corresponds to amino acid residues 1-18 from human Rab 3A.
This sequence is completely conserved between human, mouse, and rat rab 3A.
BLAST 'MASATDSRYGQKESSDQN-C' with  or 
|
|
Reacts with
(species key)
|
Hu, Ms, Rat, Dog, Hm
|
|
Tested applications
(see key)
|
ICC/IF, IHC-Fr, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: 1/200 (see Abreview)
IHC-Fr: 1/300.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Hepa1 cells
|
|
Cellular localization
|
Cell Membrane; Lipid-anchor; Cytoplasmic side
|
|
Research areas
|
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
|
|
Relevance
|
Rab3A is a 25kD GTP-binding protein implicated in neurotransmitter release. The geranylgeranyl groups at the carboxyl-terminus of Rab3A are essential for its association with synaptic vesicles, guanine nucleotide-dissociation factor (GDI), and for GTP binding. Rab3A is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis, which is in turn modulated by GTPase activating protein (GAP), GDI, guanine nucleotide-releasing factor (GRF), and rabphilin. The dif-ferent guanine nucleotide binding states of Rab3A is postulated to affect its ability to associate or dissociate with membranes during synaptic vesicle recycling. Its GTP-bound form, which represents the active form of Rab3A, associates with membrane and regulates vesicle docking and fusion. Rab3A and its homolog, Rab3C, have been shown to be co-localized to and dissoci-ated from synaptic vesicles after the stimulation of exocytosis. Deletion of the Rab3A structural gene has minimal effect on the morphology of the nervous system and spontaneous and evoked synaptic currents. However, Rab3A knockout mice exhibited that an increased rate of synaptic depression after repeated stimulation, suggesting Rab3A is involved in maintaining a steady supply of vesicles for release.
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP.
Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa.
Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Rab3A antibody images, references, products related to ab3335 and other tools.
|
| |
Rab3A antibody images:
|
 Western blot - Rab3A antibody (ab3335)
Western blot of rab 3A on Hepa1 cell lysate using ab3335
|
| |
Immunocytochemistry/ Immunofluorescence - Rab3A antibody (ab3335)
ab3335 staining hamster CHO-K1 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
|
| |
References for Rab3A antibody (ab3335)
|
|
This product has been used in: (two most recent references)
Rastaldi MP et al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J 20:976-8 (2006). PubMed: 16585060
Bernal-Mizrachi E et al. Defective insulin secretion and increased susceptibility to experimental diabetes are induced by reduced Akt activity in pancreatic islet beta cells. J Clin Invest 114:928-36 (2004). PubMed: 15467831
If you publish research using ab3335 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Rab3A
|
| |
Rab3A antibody - more information
|
Customer reviews (feedback) regarding Rab3A antibody |
Customer FAQs regarding Rab3A antibody |
Protocols for Rab3A antibody |
Price and availability of products related to Rab3A antibody |
| |
Rab3A antibody - related products:
|
|
|
| |
Rab3A antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Rab3A antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
