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Anti-Rab3A antibody (ab3335)

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Overview

Product name

Anti-Rab3A antibody
See all Rab3A products (3) ...

Description

Rabbit polyclonal to Rab3A

Tested applications

ICC/IF, IP, WB, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Dog, Human

Immunogen

Synthetic Peptide: M(1)ASATDSRYGQKESSDQN(18)C. Immunizing peptide corresponds to amino acid residues 1-18 from human Rab 3A. This sequence is completely conserved between human, mouse, and rat rab 3A.

MASATDSRYG QKESSDQN-C

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Hepa1 cells

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA, 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

  • Western blot - Rab3A antibody (ab3335)Western blot - Rab3A antibody (ab3335) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Rab3A antibody (ab3335)Immunocytochemistry/ Immunofluorescence - Rab3A antibody (ab3335) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Tissue specificity

Specifically expressed in brain.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP20336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Rab 3A antibody
  • RAB 3A member RAS oncogene family antibody
  • Rab3a antibody
  • RAB3A member RAS oncogene family antibody
  • RAB3A_HUMAN antibody
  • RAS associated protein RAB 3A antibody
  • RAS associated protein RAB3A antibody
  • Ras related protein Rab 3A antibody
  • Ras related protein Rab3A antibody
  • Ras-related protein Rab-3A antibody
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Database links

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Anti-Rab3A antibody images:

  Western blot - Rab3A antibody (ab3335)

Western blot - Rab3A antibody (ab3335)

Western blot of rab 3A on Hepa1 cell lysate using ab3335

  Immunocytochemistry/ Immunofluorescence - Rab3A antibody (ab3335)

Immunocytochemistry/ Immunofluorescence - Rab3A antibody (ab3335)

ab3335 staining hamster CHO-K1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Rab3A antibody (ab3335)

This product has been referenced in:

  • Annangudi SPet al. Neuropeptide Release is Impaired in a Mouse Model of Fragile X Mental Retardation Syndrome. ACS Chem Neurosci 1:306-314 (2010). WB; Human.Read more (PubMed: 20495672) »
  • Rastaldi MPet al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J 20:976-8 (2006).Read more (PubMed: 16585060) »

See all 3 publications for this product

Publishing research using ab3335? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"