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Western blot - Rab4 antibody (ab55656)
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Product Name
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Rab4 antibody
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See all Rab4 - Early Endosome Marker antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Rab4
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Immunogen
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Recombinant full length protein, corresponding to amino acids 1-219 of Human Rab4
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; Peripheral membrane protein. Cytoplasm. Note: Generally associated with membranes. Cytoplasmic when phosphorylated by CDC2.
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
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Relevance
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Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Rab4 antibody images:
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 Western blot - Rab4 antibody (ab55656)
Predicted band size : 24 kDa
Rab4 antibody (ab55656) at 1ug/lane + HeLa cell lysate at 25ug/lane.
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Search PubMed (MEDLINE) for references to Rab4
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Rab4 antibody - more information
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Customer reviews (feedback) regarding Rab4 antibody |
Customer FAQs regarding Rab4 antibody |
Protocols for Rab4 antibody |
Price and availability of products related to Rab4 antibody |
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Rab4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rab4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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