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Proteins and Peptides
ab88191
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab87802? Please let us know so that we can cite the reference in this datasheet
ab87802 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Rab4 antibody (ab87802)
All lanes : Anti-Rab4 antibody (ab87802) at 1 µg/ml
Lane 1 :
Lane 2 :
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 108 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - Rab4 antibody (ab87802)
ICC/IF image of ab87802 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87802, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) PC12 cells at 1µg/ml.
Western blot - Anti-Rab4 antibody (ab87802)
All lanes : Anti-Rab4 antibody (ab87802) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 100 kDa,55 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Rab4 antibody for Immunohistochemistry (PFA perfusion fixed frozen sections) in Rat (87802)
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