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SDS-PAGE - Rab4 protein (ab40228)
(enlarge)
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Product Name
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Rab4 protein (T7 Tag)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant full length protein (Human).
Fusion: T7 tag at N terminus.
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Tested applications
(see key)
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MS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
MS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This protein can be used with studies using ab13252.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
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Relevance
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Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 0.002% Sodium Azide
Constituents: 10mM Tris, 0.1% Triton X-100, pH 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
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Expression system
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E. coli
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab4 protein (T7 Tag) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab4 protein (T7 Tag) images, references, products related to ab40228 and other tools.
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Rab4 protein (T7 Tag) images:
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 SDS-PAGE - Rab4 protein (ab40228)
Analysis of RAB4A Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining shows a band around 23kDa.
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Search PubMed (MEDLINE) for references to Rab4
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Rab4 protein (T7 Tag) - more information
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