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Read our guarantee »Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
Anti-Rab5 antibody [Rab5-65]
See all Rab5 products (10) ...
Mouse monoclonal [Rab5-65] to Rab5
Indirect ELISA, ICC/IF, WBmore details
Reacts with
Mouse, Rat, Cat, Dog, Human
Predicted to work with
Chicken, Cow, Pig, Zebrafish, Cynomolgus Monkey
Synthetic peptide: ASRGATRPNGPNT conjugated to KLH, corresponding to amino acids 2-14 of Human Rab5
ASRGATRPNG PNT
Total cell extract of HeLa cells
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration information loading...
IgG fraction
Monoclonal
Rab5-65
NS1
IgG3
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
Our Abpromise guarantee covers the use of ab50523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
I-ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Array: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Required for the fusion of plasma membranes and early endosomes.
Belongs to the small GTPase superfamily. Rab family.
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
Target information above from: UniProt accessionP20339
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523)
![Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523)](/ps/datasheet/Images/50/ab50523/ab50523 ICC IF.jpg)
ICC of Hela cells, using ethanol/methanol fixation (10 min/1 min) at –20 °C. Antibody concentration 4 µg/mL. Anti-Mouse Fab-Cy3, as secondary Ab.
Western blot - Rab5 antibody [Rab5-65] (ab50523)
![Western blot - Rab5 antibody [Rab5-65] (ab50523)](/ps/datasheet/Images/50/ab50523/ab50523 WB.jpg)
Predicted band size : 24 kDa
Observed band size : 24 kDa
Hela cell extract was separated on SDS-PAGE and blotted with Anti-Rab5. Antibody concentration is 2 μg/mL. The antibody was developed with Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat and ECL substrate.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab50523? Please let us know so that we can cite the reference in this datasheet
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![Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523)](/ps/datasheet/Images/50/ab50523/ab50523 ICC IF.jpg)
ICC of Hela cells, using ethanol/methanol fixation (10 min/1 min) at –20 °C. Antibody concentration 4 µg/mL. Anti-Mouse Fab-Cy3, as secondary Ab.
![Western blot - Rab5 antibody [Rab5-65] (ab50523)](/ps/datasheet/Images/50/ab50523/ab50523 WB.jpg)
Predicted band size : 24 kDa
Observed band size : 24 kDa
Hela cell extract was separated on SDS-PAGE and blotted with Anti-Rab5. Antibody concentration is 2 μg/mL. The antibody was developed with Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat and ECL substrate.
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