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Anti-Rab5 antibody [Rab5-65] (ab50523)

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Overview

Product name

Anti-Rab5 antibody [Rab5-65]
See all Rab5 products (10) ...

Description

Mouse monoclonal [Rab5-65] to Rab5

Tested applications

Indirect ELISA, ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Cat, Dog, Human

Predicted to work with

Chicken, Cow, Pig, Zebrafish, Cynomolgus Monkey

Immunogen

Synthetic peptide: ASRGATRPNGPNT conjugated to KLH, corresponding to amino acids 2-14 of Human Rab5

ASRGATRPNG PNT

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Total cell extract of HeLa cells

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Rab5-65

Myeloma

NS1

Isotype

IgG3

Research areas

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs

  • Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523)Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523) image (enlarge)

  • Western blot - Rab5 antibody [Rab5-65] (ab50523)Western blot - Rab5 antibody [Rab5-65] (ab50523) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution.
I-ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Required for the fusion of plasma membranes and early endosomes.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Cellular localization

Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.

Target information above from: UniProt accessionP20339 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • RAB 5 antibody
  • RAB 5A antibody
  • RAB5A antibody
  • RAB5A member RAS oncogene family antibody
  • RAB5A_HUMAN antibody
  • RAS associated protein RAB5A antibody
  • Ras related protein Rab 5A antibody
  • Ras-related protein Rab-5A antibody
see all

Database links

see all

Anti-Rab5 antibody [Rab5-65] images:

  Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523)

Immunocytochemistry/ Immunofluorescence - Rab5 antibody [Rab5-65] (ab50523)

ICC of Hela cells, using ethanol/methanol fixation (10 min/1 min) at –20 °C. Antibody concentration 4 µg/mL. Anti-Mouse Fab-Cy3, as secondary Ab.

  Western blot - Rab5 antibody [Rab5-65] (ab50523)

Western blot - Rab5 antibody [Rab5-65] (ab50523)



Predicted band size : 24 kDa
Observed band size : 24 kDa


Hela cell extract was separated on SDS-PAGE and blotted with Anti-Rab5. Antibody concentration is 2 μg/mL. The antibody was developed with Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat and ECL substrate.

References for Anti-Rab5 antibody [Rab5-65] (ab50523)

This product has been referenced in:

  • Watanabe Aet al. Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation. J Biol Chem : (2011). ICC/IF; Human.Read more (PubMed: 21266579) »
  • Chamberlain MDet al. Deregulation of Rab5 and Rab4 proteins in p85R274A-expressing cells alters PDGFR trafficking. Cell Signal 22:1562-75 (2010). ICC/IF.Read more (PubMed: 20570729) »

See all 2 publications for this product

Publishing research using ab50523? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"