Rab9 antibody [Mab9] (ab2810)

Product Name 

Rab9 antibody [Mab9]

Product type 

Primary antibodies

Description 

Mouse monoclonal [Mab9] to Rab9

Immunogen 

Recombinant full length protein (Dog).

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Dog, Hm, NHuPrm

Specificity 

Recognizes prenylated and non-prenylated rab 9. This antibody does not cross-react with other rab family members.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IM, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: 1/500.
IHC-Fr: 1/500.
IM: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 26 kDa, representing Rab 9 from K562 cell extract.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane (lipid anchor; cytoplasmic side).

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome

Relevance 

RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network.

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components.
It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Mab9

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Rab9 antibody [Mab9] images, references, products related to ab2810 and other tools.

Rab9 antibody [Mab9] images:

References for Rab9 antibody [Mab9] (ab2810)

  This product has been used in: (two most recent references)

Molle D et al. Endosomal trafficking of HIV-1 gag and genomic RNAs regulates viral egress. J Biol Chem 284:19727-43 (2009). WB; Human. PubMed: 19451649

Tailleux L et al. Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. PLoS ONE 3:e1403 (2008). WB; Human. PubMed: 18167562

See all 6 publication references for this product.


If you publish research using ab2810 please let us know so that we can cite the reference on this datasheet.

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