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Product Name 

Rab9 antibody [Rab9-29]

 

See all Rab9 antibodies (4)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [Rab9-29] to Rab9

Immunogen 

Synthetic peptide: TVNLHRKPKPSSSC conjugated to KLH, corresponding to amino acids 187-200 of Human Rab9
BLAST 'TVNLHRKPKPSSSC' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Ms, Rat, Dog, Hm

Predicted to react 
(species key)

AGMk, Chk, Cow
(Due to sequence homology)

Specificity 

ab50309 recognizes RAB9.

Tested applications 
(see key)

ELISA, ICC, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 23 kDa.
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Total cell extract of HeLa cells.

Cellular localization 

Cell Membrane (lipid anchor; cytoplasmic side).

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome

Relevance 

RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network.

 

Alternative names Rab9 antibody [Rab9-29] (ab50309)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

418635    (Chicken)
403947    (Dog)
9367    (Human)
56382    (Mouse)
84589    (Rat)

Omim

    

300284    (Human)

SwissProt

    

P24408    (Dog)
P51151    (Human)
Q9R0M6    (Mouse)
Q99P75    (Rat)

Unigene

    

35289    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Rab9-29

Isotype 

IgG1

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified immunoglobulin.

Form 

Liquid

Concentration 

2.300 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Mix suspension via gentle inversion before use.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab9 antibody [Rab9-29] is on this datasheet. But please do contact us if you would like any reassurance!


 

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