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Anti-Rab9 antibody [Rab9-29] (ab50309)

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Overview

Product name

Anti-Rab9 antibody [Rab9-29]
See all Rab9 products (6) ...

Description

Mouse monoclonal [Rab9-29] to Rab9

Specificity

ab50309 recognizes RAB9.

Tested applications

ELISA, WB, IP, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Dog, Human

Predicted to work with

Chicken, Cow, African Green Monkey

Immunogen

Synthetic peptide: TVNLHRKPKPSSSC conjugated to KLH, corresponding to amino acids 187-200 of Human Rab9

TVNLHRKPKP SSSC

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Total cell extract of HeLa cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Mix suspension via gentle inversion before use.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purification notes

Purified immunoglobulin.

Clonality

Monoclonal

Clone number

Rab9-29

Isotype

IgG1

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • WB

     

  • IP

     

  • ICC

     

Application notes

ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 23 kDa.
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the transport of proteins between the endosomes and the trans Golgi network.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Cellular localization

Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.

Target information above from: UniProt accessionP51151 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 2410064E05Rik antibody
  • AI195561 antibody
  • DmRab9 antibody
  • Rab 9 antibody
  • RAB 9A antibody
  • RAB9 member RAS oncogene family antibody
  • RAB9A antibody
  • RAB9A member RAS oncogene family antibody
  • RAB9A_HUMAN antibody
  • RAS ASSOCIATED PROTEIN RAB9 antibody
  • Ras related protein Rab 9A antibody
  • Ras-related protein Rab-9A antibody
  • Sid6061p antibody
  • Sid99 antibody
see all

Database links

see all

References for Anti-Rab9 antibody [Rab9-29] (ab50309)

This product has been referenced in:

  • Ganley IGet al. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell 15:5420-30 (2004).Read more (PubMed: 15456905) »
  • Díaz Eet al. A novel Rab9 effector required for endosome-to-TGN transport. J Cell Biol 138:283-90 (1997).Read more (PubMed: 9230071) »

See all 2 publications for this product

Publishing research using ab50309? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"