Anti-Rab9 antibody (ab235538)
Key features and details
- Rabbit polyclonal to Rab9
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rab9 antibody
See all Rab9 primary antibodies -
Description
Rabbit polyclonal to Rab9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Recombinant fragment corresponding to Human Rab9 aa 100 to the C-terminus.
Database link: P51151 -
Positive control
- WB: Jurkat, K562, HepG2, HEK-293T and HeLa whole cell lysate. IHC-P: Human prostate cancer and adrenal gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. |
Target
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Function
Involved in the transport of proteins between the endosomes and the trans Golgi network. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 403947 Dog
- Entrez Gene: 9367 Human
- Entrez Gene: 56382 Mouse
- Entrez Gene: 84589 Rat
- Omim: 300284 Human
- SwissProt: P24408 Dog
- SwissProt: P51151 Human
- SwissProt: Q9R0M6 Mouse
see all -
Alternative names
- 2410064E05Rik antibody
- AI195561 antibody
- DmRab9 antibody
see all
Images
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All lanes : Anti-Rab9 antibody (ab235538) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution -
Paraffin-embedded human prostate cancer tissue stained for Rab9 with ab235538 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human adrenal gland tissue stained for Rab9 with ab235538 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab235538 has been referenced in 1 publication.
- Zhang Q et al. Circ_0013359 facilitates the tumorigenicity of melanoma by regulating miR-136-5p/RAB9A axis. Open Life Sci 16:482-494 (2021). PubMed: 34056112