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Western blot - Rab9 antibody (ab45864)
(enlarge)
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Product Name
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Rab9 antibody
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See all Rab9 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rab9
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab9. (Peptide available as ab45894.)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following tissue lysates: Spleen (Mouse) and Spleen (Rat).
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Cellular localization
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Cell Membrane (lipid anchor; cytoplasmic side).
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
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Relevance
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RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab9 antibody images, references, products related to ab45864 and other tools.
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Rab9 antibody images:
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 Western blot - Rab9 antibody (ab45864)
All lanes : Rab9 antibody (ab45864) at 1 µg/ml
Lane 1 : Spleen (Mouse) Tissue Lysate (ab29293)
Lane 2 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Rab9
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Rab9 antibody - more information
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Customer reviews (feedback) regarding Rab9 antibody |
Customer FAQs regarding Rab9 antibody |
Protocols for Rab9 antibody |
Price and availability of products related to Rab9 antibody |
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Rab9 antibody - related products:
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Rab9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rab9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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