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Rabbit Control IgG - ChIP Grade (ab46540)

Q&A (5)Specific References (20)

Overview

  • Product nameRabbit Control IgG - ChIP Grade
  • Specificityrecognizes mouse IgG heavy and light chain
  • Tested applicationsELISA, WB, ChIP more details
  • Immunogen

    Native, mouse whole IgG.

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was purified by affinity chromatography using mouse IgG-agarose.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
  • RelevanceIgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
  • Cellular localizationSecreted
    • Alternative names
        Constant region of heavy chain of IgG1G1m markerGm marker
        Ig gamma 1 chain C regionImmunoglobin heavy constant gamma 1Immunoglobulin gamma 1
      see all
  • Cellular localizationSecreted

    • Applications

    Our Abpromise guarantee covers the use of ab46540 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: 1/20000 - 1/100000.
    WB WB: 1/2000 - 1/10000. Predicted molecular weight: 40 kDa. This product is suitable for IPPT, Immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.
    ChIP ChIP: Use at an assay dependent dilution.

    Protocols

    References for Rabbit Control IgG - ChIP Grade (ab46540)

    This product has been referenced in:
    • Yin P  et al. Genome-wide progesterone receptor binding: cell type-specific and shared mechanisms in T47D breast cancer cells and primary leiomyoma cells. PLoS One 7:e29021 (2012). ChIP . Read more (PubMed: 22272226) »
    • Duverger O  et al. Neural crest deletion of Dlx3 leads to Major Dentin defects through downregulation of Dspp. J Biol Chem : (2012). ChIP . Read more (PubMed: 22351765) »

    See all 20 Publications for this product

    Publishing research using ab46540 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab46540

    Displaying 1 - 5 of 5 results for Q&A

    10
    Votes: Highest First

    I need some help designing my ChIP experiment.

    Thank you for contacting us.


    First, I'd like to point out some resources on our website which may help:


    http://www.abcam.com/index.html?rid=310


    A Beginner's Guide to ChIP - Abcam


    As we discus...

    Read More

    phone call about rabbit IGG controls for ChIP

    Merci beaucoup pour votre appel de ce matin.

    Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouv&eac...

    Read More

    Hello,

    I'm using Rabbit Control IgG - ChIP Grade (ab46540)
    for my chip experiment.
    Do you have equivalent for rat and mouse? I don't find them,


    Thanks in advance,

    Thank you for your inquiry.

    Indeed we do have also polyclonal control fractions of mouse or rat IgGs.

    For the mouse IgG, we do have the IgG fraction ab37355 http://www.abcam.com/index.html?datasheet=37355 (or use the following: http://...

    Read More

    dear correspondent,

    We have lately purchased several Abs from Abcam and i have a question related to the purification method.
    In the datasheets of several monoclonal antibodies is mentioned that they are purified as 'IgG fraction'. Could y...

    Read More

    Thank you for your enquiry.

    I have confirmed the purification details of the antibodies you listed and have updated the datasheets where necessary:

    ab3609 (Aurora B)
    Protein G purified

    ab51841 (TATA binding protein) <...

    Read More

    Dear, We lately bought the rabbit control IgG antibody. We would like to rebuy it but need further information on the following question. Is this antibody purified via protein A chromatography or not? This is important information for us if we want to ass...

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    Thank you for your inquiry.
    I can confim that the antibody was affinity-purified against the antigen itself (bound to agarose).
    Protein A or G was not used.
    I hope this information is helpful. please do not hesitate to contact me with any f...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"