Rabbit Anti-Goat IgG+IgM+IgA H&L (HRP) (ab8515)
Key features and details
- Rabbit Anti-Goat IgG+IgM+IgA H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: ELISA, Dot blot
Overview
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Product name
Rabbit Anti-Goat IgG+IgM+IgA H&L (HRP)
See all IgG + IgM + IgA secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Specificity
Antibodies were raised in rabbit to goat IgG, IgM and IgA -
Tested applications
Suitable for: ELISA, Dot blotmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Specific antibodies were absorpted by incubation with Sepharose-bound goat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized goat immunoglobulins a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/1000.
|
|
Dot blot |
1/100.
|
Notes |
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ELISA
1/1000. |
Dot blot
1/100. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab8515 has been referenced in 1 publication.
- Wall VZ et al. Inflammatory stimuli induce acyl-CoA thioesterase 7 and remodeling of phospholipids containing unsaturated long (=C20)-acyl chains in macrophages. J Lipid Res 58:1174-1185 (2017). PubMed: 28416579