Overview

  • Product nameRabbit IgG- ChIP Grade
    See all Rabbit isotype controls
  • SpecificityThis antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
  • Tested applicationsELISA, Flow Cyt, ChIP/Chipmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesProtein A-purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
  • Alternative names
    • rabbit isotype control

Applications

Our Abpromise guarantee covers the use of ab37415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
ChIP/Chip Use at an assay dependent dilution. PubMed: 20673990

References for Rabbit IgG- ChIP Grade (ab37415)

This product has been referenced in:
  • Marion-Poll L  et al. Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications. Eur J Neurosci 39:1234-44 (2014). Flow Cyt . Read more (PubMed: 24713002) »
  • Zhiponova MK  et al. Helix-loop-helix/basic helix-loop-helix transcription factor network represses cell elongation in Arabidopsis through an apparent incoherent feed-forward loop. Proc Natl Acad Sci U S A 111:2824-9 (2014). IP . Read more (PubMed: 24505057) »

See all 21 Publications for this product

Product Wall

The recommended isotype control for use with ab86386 Rabbit polyclonal to HOXB2 is ab37415 Rabbit IgG- ChIP Grade .

Thank you for contacting us.

ab37415 has been tested in flow cytometry as Isotype control.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbi...

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Thank you for your enquiry.

I can confirm that ab134080Anti-CD30 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentrat...

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Thank you for calling Abcam earlier today.

Please find attached the Abcam protocols book that we discussed on the telephone.

Also we do have a few ChiP kits in our catalogue that may be of interest to you:

http://www.abcam.co...

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Merci beaucoup pour votre appel de ce matin.

Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouv&...

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Thank you for your reply.

The only other phospho-smad3 (S423+S425) we have are:

ab11825 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody-ab118825.html

ab52903 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody...

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Thank you very much for contacting Abcam.

The isotype control that I would recommend for your flow cytometry experiments is:

http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



Below is the infromation about...

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Thank you for your inquiry.

The best way to find isotype controls for an antibody is to start at our homepage www.abcam.com. Go to the yellow bar at the top of the page and move the mouse to “Products,” then click on “Iso...

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Thank you for contacting us. Please check ab37415: http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html This antibody does not have BSA and sodium azide in it. For ab27472 and and27478 the conc. of azide is very low so theoreticall...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"