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Rabbit IgG FITC - Isotype Control (ab37406)

Q&A (13)Specific References (1)

Overview

  • Product nameRabbit IgG FITC - Isotype ControlSee all Rabbit isotype controls ...
  • ConjugationFITC
  • SpecificityThis antibody has been selected to be an isotype control as it has no known specificty.
  • Tested applicationsELISA, Flow Cyt more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 100mM Tris buffered saline, pH 8
  • Concentration information loading...
  • PurityIgG fraction
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
    • Alternative names
        rabbit isotype control

    Applications

    Our Abpromise guarantee covers the use of ab37406 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    Flow Cyt Flow Cyt: Use 10µl for 106 cells.

    References for Rabbit IgG FITC - Isotype Control (ab37406)

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab37406 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab37406

    Displaying 1 - 10 of 13 results for Q&A

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    Inquiry: I have purchased the product with details below. Can you please provide me the concentration and the volume of the the product? What is your recommended concentration to use for flow cytometry using frozen peripheral blood mononuclear cell (PBMC)...

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    Thank you for contacting us and your interest in our products.

    The Rabbit IgG FITC - Isotype Control (ab37406) of the lot number GR59579-1 is provided at a concentration of 0.1mg/mL and is supplied as 1 mL.

    As the antibody is intended ...

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    Hi,

    I have purchased ab37406 lot number GR77642. I need to use this alongside other antibodies at a known concentration.

    Please could you tell me the concentration of this antibody.

    Regards,

    Thank you for your inquiry.
    I am happy to confirm that ab37406 (lot GR77642) has a concentration of 0.1mg/ml.
    I hope this information is helpful and wish you good luck with your research.

    For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them.
    ab36204 --> Rabbit IgG
    ab48614 --> Rabbit IgG
    ab66219 --> Rab...

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    Thank you for your clarification.

    I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I c...

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    Thank you for the reply.
    In answer to your questions:
    1. Unfortunately I have no data supporting the expression levels of
    the CDT1 in this particular cell line so it's definitely worthwhile to
    stain HeLa cells and see whe...

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    I think its good that you are checking the cell viability and using HeLa may provide more information as to if it is the cell strain or the antibodies that may be contributing to the problems. I agree that checking both the primary and secondary antibody ...

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    Hi xxxx,

    Firstly I'd like to thank you for all the suggestions you've made so far, it's been a very nice Abcam experience and the better technical support I've ever got. So, thank you again.

    You are right, now we now that there is de...

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    Thank you for providing such a full explanation of what you are trying to achieve. I can now understand exactly why you have chosen the technique that you have.

    I think the experiments you are planning to do next will be very useful in determini...

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    Dear xxxx,

    I contacted you a few weeks ago as I'm having having some problems for stainng my samples with the following ICC system:
    Primary antibody: Anti-CDT1 antibody (ab83174)
    Secondary Antibody: Goat polyclonal Secondary Antibody ...

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    Thank you for getting back to me with your new results. It is good that we can now say conclusively that there is non-specific binding of both the secondary antibody and of the isotype control antibody.

    Following the protocol changes, I do not e...

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    Dear xxxx,

    The information provided is much appreciated, thank you very much for all the suggestions made. I think that definitely there are opportunities for improving my protocol.
    In answer to your question, the preparation of the dilute...

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    Thank you for that last bit of information.


    I just wanted to check that the isotype control wasn't being used in a higher concentration than the primary antibody, but everything is fine.


    I look forward to hearing how you ge...

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    Dear xxxx,

    Please find attached to this email the images corresponding to both, the positve staining controls and the actual controls for discarding misbinding of the antibodies.
    Even thouh I'm showing just one set of images the general tr...

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    Sorry for the delay in getting back to you and thank you for all that information. It makes it much easier to understand what is going on and what has been tried previously.

    I think the next step you are suggestingare good. The blocking buffer a...

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    Phone call requestion advice on results obtained from ICC isotype control experiment using ab37406.

    Thank you for contacting us and reporting the problems you have encountered in using the Anti-CDT1 antibody (ab83174) and rabbit IgG isotype control (ab37406).


    As discussed over the phone, in order to provide the most suitable suggestions ...

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    Using:
    ab37406 FITC conjugated rabbit isotype control
    Rabbit primary antibody
    ab60314 Goat antirabbit secondary
    Obtaining high background in ICC from the isotype control.

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the isotype control, ab37406.

    I would like to reassure you that this isotype control is tested and covered by our 6 mont...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"