Rabbit IgG secondary antibody - H&L - Pre-Adsorbed (ab7088)

Product Name 

Rabbit IgG secondary antibody - H&L - Pre-Adsorbed

Product type 

Secondary antibodies

Description 

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (TR)

Immunogen 

Full length native Rabbit IgG (purified).

Species Reactivity 

Minimum reactivity to Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IM

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Rabbit >> IgG >> Fluorophore >> Texas Red
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.

Form 

Liquid

Conjugation 

Texas Red ®

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Rabbit IgG secondary antibody - H&L - Pre-Adsorbed (ab7088)

  This product has been used in: (two most recent references)

Ho G et al. Molecular imaging of retinal gliosis in transgenic mice induced by kainic acid neurotoxicity. Invest Ophthalmol Vis Sci 50:2459-64 (2009). PubMed: 19151398

Ho G et al. Non-invasive fluorescent imaging of gliosis in transgenic mice for profiling developmental neurotoxicity. Toxicol Appl Pharmacol 221:76-85 (2007). PubMed: 17350065
If you publish research using ab7088 please let us know so that we can cite the reference on this datasheet.

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