Goat anti-Rabbit IgG H&L (Chromeo™ 546) secondary antibody (ab60317)
Overview
- Product nameGoat anti-Rabbit IgG H&L (Chromeo™ 546) secondary antibodySee all IgG secondary antibodies ...
- DescriptionGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 546)
- Target speciesRabbit
- Specificityab60317 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
- Tested applicationsICC/IF more details
- Immunogen
Rabbit IgG.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 2mM Sodium Azide
Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Conjugation notesChromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo™ 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax) Quantum Yield: ~ 13 % Excitation Wavelength Range: 530 to 550 nm Emission Wavelength Range: 560 to 570 nm
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab60317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF |
Plate-based assays: 1/800 to 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat anti-Rabbit IgG H&L (Chromeo™ 546) secondary antibody (ab60317)
This product has been referenced in:
- Molnár T et al. Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate. BMC Neurosci 12:96 (2011). IHC . Read more (PubMed: 21967230) »
- Molnár T et al. Activation of astroglial calcium signaling by endogenous metabolites succinate and gamma-hydroxybutyrate in the nucleus accumbens. Front Neuroenergetics 3:7 (2011). ICC/IF . Read more (PubMed: 22180742) »
