Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed (ab6564)

Hi,

I'm interested in the following antibodies for IHC/IF, and I'm wondering if you could tell me if any preliminary experiments have been done on these antibodies and if the results suggest that they are good for both IHC (on FFPE) and IF?

Ab25644, ab24941, ab86900, ab41467, ab60077, ab25175, ab24985, ab112470, ab61219, ab6564, ab59437, ab116223

If they haven't been tested previously, is there a testing discount that we can have for using it in our experiments?

In addition, as we are a new lab based in ####, is there a discount which we can have for bulk ordering antibodies and other reagents from Abcam? And perhaps a discount from shipping all the reagents at all instead of individual shipping rates?

Many thanks,

ANSWER:

Thank you very much for your interest in Abcam and our products..

To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated as soon as we receive any new information.

ab25644
ab24941
ab86900
ab41467,
ab60077,
ab25175,
ab24985,
ab59437,
ab116223

Therefore, I can offer a discount off a future purchase if you buythese untested antibodies now, test in IHC-P and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback.One discount code would be provided for each individualantibody thatwould be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed.If you could let me know whichparticular antibodies youwant to test. I will then send a discount code for each of these antibody. This code must be issued before purchasing the antibodiesso please wait for my reply before ordering.

2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the any of the antibodiesturns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

The following antibodies you listed have already been tested in IHC-P, and so we are not able to provide a testing discount for these on this occasion. As they have been tested in IHC-P, we can guarantee them for fluorescent studies on paraffin embedded tissue as well.

ab112470
ab61219
ab6564

We do not charge individual shipping for each item. We would charge one shipping fee for eachorder. Extra shipping charges are applied if there is a bulky item such as a kit, or a dry ice item on the order.

With regards to discounts for new laboratories, I have spoken to our marketing department and believe thatone ofyour colleagues, Dr. ##### has already been in touch with us. A 25% discount has been arranged for orders from his laboratory for the next 1 month. Could you confirm that you are working in the same laboratories? I hope this discount will prove helpful in setting up the new laboratory. As we have already arranged these 2 types of discount, I am sorry we are not able to offer a further bulk discount or shipping discount on this occasion.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Can you please confirm whether SAP102 -ab 83980 - is compatible with this secondary:  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed (ab6564).  

ANSWER:

Yes, the secondary antibody to Rabbit IgG - H&L (Cy5 ®) ab6564 is compatible with the anti-SAP102 antibody ab83980.

Does Cy5 colocalize with Fitc? With Rhodamine? With Texas Red?

ANSWER:

Thank you for your enquiry. Cy5 emits the color red (it is located at the Far-Red end of the spectrum). The following link on our website should be helpful for you (there is a Fluorochrome table on that particulat page): http://abcam2/index.html?pageconfig=tech&mode=choosing

So, if Cy5 overlaps with Fitc, the color seen would be yellow.

Hi, I am looking for an Rb antibody to use for immunohistochemistry. I can't figure out what the 'Fr' after IHC-Fr stands for and I don't want to purchase the antibody until I am entirely sure that it will work for my purposes. I would like to order it today or tomorrow.

I am going to be staining mouse skeletal muscle sections with the Rb rabbit polyclonal primary (catalog #ab6075 ) and the secondary: ab6564 Goat polyclonal to Rabbit IgG H&L (also curious what H&L stands for) Thank you!

ANSWER:

Thank you for your email. Ab6075 has been tested for application in IHC-Frozen sections (IHC-Fr). It has not yet been tested for application in IHC-formalin fixed paraffin embedded tissue sections. For ab6564, Goat polyclonal to Rabbit IgG H&L (Cy5), H&L stands for heavy and light chain. If you have any more questions, please contact us again.

Can you tell me what colour the Cy5 will appear in histostaining please?

ANSWER:

Thank you for your recent technical enquiry.

The color of the stained signal with this antibody should be in the far red range (Excitation: 650 nm, Emission: 667 nm).

If you are interested more details on the color of some widely used fluorochromes (fluorescent labels), then please visit the following website:

http://www.abcam.com/index.html?pageconfig=tech&mode=choosing