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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)
See all IgG products (1691) ...
Target species
Rabbit
Tested applications
Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
Immunogen
Rabbit IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Immunocytochemistry/ Immunofluorescence - Rabbit IgG antibody - H&L (ab6717)
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Flow Cytometry - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab6717)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab6717)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent concentration.
Flow Cyt: 1/500 - 1/2500.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
ICC/IF: 1/1000 - 1/5000.
ELISA: 1/10000 - 1/50000.
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) images:
Immunocytochemistry/ Immunofluorescence - Rabbit IgG antibody - H&L (ab6717)
ab11320 (Rabbit polyclonal to gamma Tubulin - Centrosome Marker) at 1/400 dilution staining human neuroblastoma cells by ICC/IF. The cells were fixed with paraformaldehyde and blocked with serum and then incubated with the primary antibody for 16 hours. ab6717 was used as the secondary.
This image is courtesy of an anonymous Abreview
Flow Cytometry - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab6717)
Ab6717 was used with the primary antibody ab66506 in Flow Cyt. See Abreview on ab66506.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab6717)
Ab6717 was used at dilution 1/200 with the primary antibody ab36823 in IHC-P. See the review on ab36823.
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab6717)
This product has been referenced in:
- Liang XHet al. Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice. Nucleic Acids Res 39:e13 (2011). ICC/IF.Read more (PubMed: 21062825) »
- Grimm MOet al. Docosahexaenoic Acid Reduces Amyloid {beta} Production via Multiple Pleiotropic Mechanisms. J Biol Chem 286:14028-39 (2011). Flow Cyt.Read more (PubMed: 21324907) »
See all 26 publications for this product
Publishing research using ab6717? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"