Rabbit IgG secondary antibody - H&L (ab6722)

Product Name 

Rabbit IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Goat polyclonal to Rabbit IgG - H&L (AP)

Immunogen 

Rabbit IgG whole molecule

Tested applications 
(see key)

Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, WB

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent concentration.
ELISA: 1/1000 (see PMID 19008226).
ICC/IF: 1/2000(from PubMed: 17540569). .
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, 10mg/ml BSA, 0.05M Tris chloride, 0.15M Sodium chloride, 0.001M Magnesium chloride, 0.001M Zinc chloride, pH 8.0

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Form 

Liquid

Conjugation 

Alkaline Phosphatase

Concentration 

Batch dependent within range: 0.55 - 1.00 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C. Do not freeze.

References for Rabbit IgG secondary antibody - H&L (ab6722)

  This product has been used in: (two most recent references)

Kalamajski S & Oldberg A Homologous sequence in lumican and fibromodulin leucine-rich repeat 5-7 competes for collagen binding. J Biol Chem 284:534-9 (2009). I-ELISA. PubMed: 19008226

Follenzi A et al. Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice. J Clin Invest 118:935-45 (2008). ELISA. PubMed: 18274668

See all 4 publication references for this product.


If you publish research using ab6722 please let us know so that we can cite the reference on this datasheet.

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