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Product name
Sheep anti-Rabbit IgG H&L (HRP) secondary antibody
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Description
Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Target species
Rabbit
Tested applications
WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Frmore details
Immunogen
Rabbit IgG, whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Horseradish Peroxidase (HRP).
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Western blot - Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6795)
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Applications
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Our Abpromise guarantee covers the use of ab6795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IM: Use at an assay dependent concentration.
Dot: Use at an assay dependent concentration.
ICC/IF
ICC/IF: 1/500 - 1/2500.ICC/IF: 1/500 - 1/2500.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
Sheep anti-Rabbit IgG H&L (HRP) secondary antibody images:
Western blot - Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6795)
Ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.
References for Sheep anti-Rabbit IgG H&L (HRP) secondary antibody (ab6795)
This product has been referenced in:
- Abedin SA et al. Elevated NCOR1 disrupts a network of dietary-sensing nuclear receptors in bladder cancer cells. Carcinogenesis 30:449-56 (2009). WB.Read more (PubMed: 19126649) »
- Gribaudo S et al. Expression and localization of the calmodulin-binding protein neurogranin in the adult mouse olfactory bulb. J Comp Neurol 517:683-94 (2009). WB.Read more (PubMed: 19827160) »
See all 3 publications for this product
Publishing research using ab6795? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"