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Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6795)

Overview

  • Product nameSheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Tested applicationsWB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Fr more details
  • Immunogen

    Rabbit IgG, whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesHorseradish Peroxidase (HRP).
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution.
ELISA ELISA: Use at an assay dependent dilution.
Immunomicroscopy IM: Use at an assay dependent concentration.
Dot Blot Dot: Use at an assay dependent concentration.
ICC/IF ICC/IF: 1/500 - 1/2500.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.

Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) images

  • Ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.

References for Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6795)

This product has been referenced in:
  • Abedin SA  et al. Elevated NCOR1 disrupts a network of dietary-sensing nuclear receptors in bladder cancer cells. Carcinogenesis 30:449-56 (2009). WB . Read more (PubMed: 19126649) »
  • Gribaudo S  et al. Expression and localization of the calmodulin-binding protein neurogranin in the adult mouse olfactory bulb. J Comp Neurol 517:683-94 (2009). WB . Read more (PubMed: 19827160) »

See all 3 Publications for this product

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The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not te...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"