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Chicken polyclonal Secondary Antibody to Rabbit IgG - H&L (TRITC ) (ab6826)

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Overview

  • Product nameChicken polyclonal Secondary Antibody to Rabbit IgG - H&L (TRITC )See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Tested applicationsImmunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA more details
  • Immunogen

    Rabbit IgG, whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notesTetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notessuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    • References for Chicken polyclonal Secondary Antibody to Rabbit IgG - H&L (TRITC ) (ab6826)

    ab6826 has not yet been referenced specifically in any publications.

    Publishing research using ab6826 ? Please let us know so that we can cite the reference in this datasheet

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    ORDER NUMBER 114090 DESCRIPTION OF THE PROBLEM weak Non-specific staining SAMPLE Hela PRIMARY ANTIBODY ab2893 DETECTION METHOD fluorescent microscoop POSITIVE AND NEGATIVE CONTROLS USED pos control radiation with UV during 45 mi...

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    I'm sorry to hear you are experiencing problems with ab6826, the fact that a "no primary" control gave you high background does indeed point towards a non-specificity of a damaged vial. Could you please clarify what dilution of this secondary antibody you...

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    I know it says purified IgG, but has it been affinity purified so that it does not cross react with other species. The antibody will be mixed with donkey, goat and other secondary antibodies

    All the information avaliable for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose". Therefore this antibody will be ...

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    Hi, there, I am gonna do the immunostaining on the human cell lines, the primary antibody I have now was raised in rabbit, and I need a secondary anti-rabbit antibody conjugated with rhogamine, on your list, there are so many secondary antibodis fit my...

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    There should be no difference between any of these secondaries; they will all work similarly well. However, if you wish to reduce background with the human cells then we suggest using secondaries that have been pre-adsorbed against human serum, thus, G...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"