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Product Name
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Rabbit IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Goat polyclonal to Rabbit IgG - H&L (Agarose)
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Immunogen
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Rabbit IgG whole molecule
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Tested applications
(see key)
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AP, IHC-Fr, IHC-P, IP
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Application notes
(see key)
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Recommended dilutions
Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure rabbit IgG.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Cellular localization
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Secreted
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Research areas
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Tags & Cell Markers >> Epitope Tags >> Agarose Conjugates
Secondary antibodies >> anti-Rabbit >> IgG >> Agarose
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies and proteins, including recombinant fusion proteins, from a complex protein mixture (see protocol).
Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.
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Rabbit IgG secondary antibody - H&L (ab7019) |
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Raised in
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Goat
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Coupled to cyanogen bromide activated cross-linked 4% agarose.
10.0 mg antibody per cc agarose
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerosal, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product is an IgG fraction antibody coupled to activated agarose.
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Form
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Liquid
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Conjugation
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Agarose
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rabbit IgG secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to IgG
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