Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbed (ab27237)
Overview
- Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRabbit
- Tested applicationsElectron Microscopy more details
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Minimal
cross-reactivity
Human more details - Immunogen
Full length native Rabbit IgG
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 20% Glycerol, 1% BSA, Tris buffered saline. pH 8.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab27237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Electron Microscopy |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbed images
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Electron Microscopy - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbed (ab27237)Image from Berger M et al, J Biol Chem. 2012 Jun 1;287(23):19409-17. Epub 2012 Apr 24, Fig 3. DOI 10.1074/jbc.M112.353557 June 1, 2012 The Journal of Biological Chemistry, 287, 19409-19417.ab27237 used in Electron Microscopy.Subcellular localization of RIG-I in human neutrophils.C: Isolated peripheral blood neutrophils were fixed, washed with PBS, permeabilized, and blocked (10% normal goat serum, 10 mm glycine, and 0.2% Tween 20 in diluent containing 0.5% bovine serum albumin and 0.5% Triton X-100 in PBS). For visualization of RIG-I, the grids were incubated with anti-RIG-I antibody and a gold-conjugated secondary antibody (gold sphere diameter, 5 nm; ab27237, Abcam). Finally, the grids were stained with 1% uranyl acetate. Negative controls were obtained by omitting the primary antibody.Arrows indicate RIG-I staining. The red arrows mark RIG-I associated with a vesicle-like structure.
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbed (ab27237)
This product has been referenced in:
- Berger M et al. Neutrophils Express Distinct RNA Receptors in a Non-canonical Way. J Biol Chem 287:19409-17 (2012). Electron Microscopy . Read more (PubMed: 22532562) »
