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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Goat anti-Rabbit IgG H&L (Chromeo™ 488) secondary antibody
See all IgG products (1691) ...
Description
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488)
Target species
Rabbit
Specificity
ab60314 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
Tested applications
ICC/IFmore details
Immunogen
Rabbit IgG.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 2mM Sodium Azide
Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Conjugation notes
Chromeo™ 488 is spectraly similar to FITC and Alexa® 488. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 490 and 527 nm which are compatible with common excitation sources and filter sets. Molar Extinction Coefficient: 73,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 52 % Excitation Wavelength Range: 470 to 500 nm Emission Wavelength Range: 515 to 530 nm
Clonality
Polyclonal
Isotype
IgG
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Fluorophore >> Chromeo™ 488
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Immunocytochemistry/ Immunofluorescence - Rabbit IgG antibody (ab60314)
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- Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab60314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/1000 - 1/2000.(Plate-based assays: 1/800 - 1/1000.)
Goat anti-Rabbit IgG H&L (Chromeo™ 488) secondary antibody images:
Immunocytochemistry/ Immunofluorescence - Rabbit IgG antibody (ab60314)
ICC/IF imaging of anti-tubulin in U2OS cells stained with propidium iodide and incubated with rabbit anti-tubulin antibody. Ab60314 was then used at 1:1000. The cells were previously fixed and permeabilized by methanol.
- Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
Ab60314 was used at dilution 1/500 with the primary antibody ab18532 in IHC - Free Floating. See the review on ab18532.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
Ab60314 was used at dilution 1/1000 with the primary antibody ab24445 in IHC-FrFl. See the review on ab24445.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
Ab60314 was used at dilution 1/500 with the primary antibody ab32787 in IHC-P. See the review on ab32787.
Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
Ab60314 was used at dilution 1/500 with the primary antibody ab48050 in IHC-Fr. See the review on ab48050.
References for Goat anti-Rabbit IgG H&L (Chromeo™ 488) secondary antibody (ab60314)
This product has been referenced in:
- Milicevic NMet al. Role of CCL19/21 and its possible signaling through CXCR3 in development of metallophilic macrophages in the mouse thymus. Histochem Cell Biol 135:593-601 (2011). IHC-P.Read more (PubMed: 21611855) »
See 1 publication for this product
Publishing research using ab60314? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"