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Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)

Submit an Abreview Q&A (13)Specific References (1)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Specificityab60314 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IF more details
  • Immunogen

    Rabbit IgG.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesChromeo™ 488 is spectraly similar to FITC and Alexa® 488. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 490 and 527 nm which are compatible with common excitation sources and filter sets. Molar Extinction Coefficient: 73,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 52 % Excitation Wavelength Range: 470 to 500 nm Emission Wavelength Range: 515 to 530 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/1000 - 1/2000.
Plate-based assays: 1/800 - 1/1000.

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) images

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG antibody (ab60314)
    Immunocytochemistry/ Immunofluorescence - Rabbit IgG antibody (ab60314)

    ICC/IF imaging of anti-tubulin in U2OS cells stained with propidium iodide and incubated with rabbit anti-tubulin antibody. Ab60314 was then used at 1:1000. The cells were previously fixed and permeabilized by methanol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)
    Ab60314 was used at dilution 1/500 with the primary antibody ab32787 in IHC-P. See the review on ab32787.

References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314)

This product has been referenced in:
  • Milicevic NM  et al. Role of CCL19/21 and its possible signaling through CXCR3 in development of metallophilic macrophages in the mouse thymus. Histochem Cell Biol 135:593-601 (2011). IHC-P . Read more (PubMed: 21611855) »

See 1 Publication for this product

Publishing research using ab60314 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab60314 Submit a Question for ab60314

Displaying 1 - 10 of 13 results for Abreviews and Q&A

10
Votes: Highest First

Customer inquiring about storage conditions for 15041 (short and long term). How long is the antibody stable under these conditions?

We store the antibody at 4°C . At the concentration of 2 mg/ml the conjugate is stable for a long period of time, though the duration of stability is unknown. We tested antibodies after more than two years in the fridge and did not recognize a differe...

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Thank you for the attention,
we have tried to use others primary antibodies and the results are always the same, so I accept your proposal to replace the antibodies,
could I know approximate consignment date of new antibodies?

thank y...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these secondary antibodies did not perform as stated on the datasheet and for the inconve...

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i have attached the completed questionaire

Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand...

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Hello,
I'm Annunziata Crupi, PhD Student in DAHFMO, histology and medical embriology unit, La Sapienza, Rome, laboratory of M. Bouchè,
I have some problems with two abcam conjugated antibodies and I have attached a file that explain every...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these two secondary antibodies.

I appreciate the time you have spent in the laboratory and understand your concerns. It ...

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Hello

Please can I have the following antibody as an alternative:
http://www.abcam.com/Rabbit-IgG-secondary-antibody-ab60314.html
Kind regards,

As requested, I have issued a free of charge replacement with the order number XXXXX for the antibody ab60314.

To check the status of the order please contact our Customer Service team and reference this number.

I hope this new vial wo...

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Hi!
Thank you for your answer.
Now I have fixed my choice on a primary antibody, I need a secondary.
I have always used Alexa-conjugated secondaries so I have no experience of the DyLight and Chromeo-conjugates that you offer. Both exhibit ...

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Thank you for contacting us.

The fluorochromes which are sold under the brand names Alexa, DyLight or Chromeo are designed to ensure an optimal performance and thus are chemically almost identical. As you may have noticed the advantagesfor these...

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Thank you for the reply.
In answer to your questions:
1. Unfortunately I have no data supporting the expression levels of
the CDT1 in this particular cell line so it's definitely worthwhile to
stain HeLa cells and see whe...

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I think its good that you are checking the cell viability and using HeLa may provide more information as to if it is the cell strain or the antibodies that may be contributing to the problems. I agree that checking both the primary and secondary antibody ...

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Hi xxxx,

Firstly I'd like to thank you for all the suggestions you've made so far, it's been a very nice Abcam experience and the better technical support I've ever got. So, thank you again.

You are right, now we now that there is de...

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Thank you for providing such a full explanation of what you are trying to achieve. I can now understand exactly why you have chosen the technique that you have.

I think the experiments you are planning to do next will be very useful in determini...

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Dear xxxx,

I contacted you a few weeks ago as I'm having having some problems for stainng my samples with the following ICC system:
Primary antibody: Anti-CDT1 antibody (ab83174)
Secondary Antibody: Goat polyclonal Secondary Antibody ...

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Thank you for getting back to me with your new results. It is good that we can now say conclusively that there is non-specific binding of both the secondary antibody and of the isotype control antibody.

Following the protocol changes, I do not e...

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Dear xxxx,

The information provided is much appreciated, thank you very much for all the suggestions made. I think that definitely there are opportunities for improving my protocol.
In answer to your question, the preparation of the dilute...

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Thank you for that last bit of information.


I just wanted to check that the isotype control wasn't being used in a higher concentration than the primary antibody, but everything is fine.


I look forward to hearing how you ge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"