Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7077)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
Overview
-
Product name
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Full length native Mouse IgG (purified).
-
Conjugation
Alkaline Phosphatase
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/500.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
ELISA |
1/9800.
|
|
IHC-P |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
WB |
1/500 - 1/2000.
|
Notes |
---|
ICC/IF
1/100 - 1/500. |
Dot blot
Use at an assay dependent dilution. |
ELISA
1/9800. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/500 - 1/2000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab7077 has not yet been referenced specifically in any publications.