Rabbit Anti-Sheep IgG H&L (Glucose Oxidase) (ab136721)
Key features and details
- Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)
- Conjugation: Glucose Oxidase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WB
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Sheep -
Tested applications
Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WBmore details -
Immunogen
Highly purified whole immunoglobulin from Sheep.
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Conjugation
Glucose Oxidase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/10 - 1/100.
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IHC-Fr |
1/10 - 1/100.
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ELISA |
1/100 - 1/1000.
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ICC |
1/10 - 1/100.
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WB |
1/400.
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Notes |
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IHC-P
1/10 - 1/100. |
IHC-Fr
1/10 - 1/100. |
ELISA
1/100 - 1/1000. |
ICC
1/10 - 1/100. |
WB
1/400. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136721 has not yet been referenced specifically in any publications.