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Overview

  • Product nameRabbit polyclonal IgGSee all Rabbit isotype controls ...
  • Specificityab27472 does not react with any antigen of human or animal cells.
  • Tested applicationsIHC-P, IP, Sandwich ELISA more details
  • Species reactivity
    ab27472 does not react with any species.
  • General notesThe concentration of this prediluted antibody is 2 µg/ml.

    • Properties

  • FormPrediluted
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab27472 is an affinity purified IgG. It was purified from serum collected from a rabbit prior to immunization.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
    • Alternative names
        rabbit isotype control

    Applications

    Our Abpromise guarantee covers the use of ab27472 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution. Use neat for 10 minutes at room temperature. Pretreatment: same as primary antibody.
    IP IP: Use at an assay dependent dilution. PubMed: 21088000
    Sandwich ELISA sELISA: Use at an assay dependent dilution. PubMed: 19243500

    References for Rabbit polyclonal IgG (ab27472)

    This product has been referenced in:
    • Mariani M  et al. The selective vitamin D receptor agonist, elocalcitol, reduces endometriosis development in a mouse model by inhibiting peritoneal inflammation. Hum Reprod 27:2010-9 (2012). Read more (PubMed: 22588001) »
    • Schmidt EP  et al. The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis. Nat Med : (2012). Read more (PubMed: 22820644) »

    See all 6 Publications for this product

    Publishing research using ab27472 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab27472

    Displaying 1 - 10 of 20 results for Q&A

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    To whom it concerns,

    Could I get a price for Rabbit polyclonal IgG (ab27472)?

    Thanks,

    Thank you for contacting us.

    The price for Ireland is €360 + €38 delivery charges.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Hello, I have ordered Anti-IL6 antibody (ab6672) to use as a neutralizing agent. I was just wondering what normal rabbit IgG you would recommend as a control for this (for use in tissue culture). Would ab27472 be the best?


    I think ab27472 that you have selected is a great isotype control for ab6672 as it is a rabbit polyclonal IgG and ab6672 is also a rabbit polyclonal with isotype IgG.

    We are looking for a CRM1 antibody and a control IgG for IHC in FFPE slides. I found three CRM1 antibodies on your website (ab24189, ab77977, and ab84375). Could you please recommend which antibody is the best for IHC? I plan to use it for tissue array.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any anti-CRM1 antibody tested in the species and application you are using sh...

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    Could you update me about my questions about CRM1 antibody?

    Thank you for your enquiry. I wish to sincerely apologize for the delay.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Th...

    Read More

    I forgot to mention that I am going to use them for flow cytometry (on LSR II).

    Thank you for your reply.
    Below are the 2 products that I would recommend for you:
    Isotype control - http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
    Secondary - http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-...

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    I am looking for an isotype control and a conjugated secondary antibody fora this primary antibody of Cyclin E, http://www.abcam.com/Cyclin-E-antibody-ab7959.html It seems that you have the following relevant products for instance: http://www.abcam.com/Ra...

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    Thank you for contacting Abcam.
    I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommend p...

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    Could we have concentrated form of ab27472?

    Thank you for contacting us.

    The concentrated form of ab27472 is ab27478.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Ab...

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    vielen Dank für Ihre schnelle Antwort. Wir werden den von Ihnen empfohlenen Isotyp bestellen.
    Im Rahmen der Antikörperbestellung möchten wir auch eine Positivkontrolle für den Westernblot (in Form eines Lysats) mitbestellen.
    ...

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    Vielen Dank für Ihre Anfrage.

    Für ab111915 wurden 20 µg Lysat aufgetragen, für ab66106 waren es10 µg (siehe Datenblatt). Normalerweise sind - je nach Expressionslevel in der Positivkontrolle - 20 bis maximal 50 µ...

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    für folgende Antikörper möchte ich eine entsprechende Isotypkontrolle bestellen, um diese bei der Färbung mitzuführen:
    Anti-IL9 antibody (https://secure.abcam.com/index.html?datasheet=111915)
    Anti-IL9R antibody (https://...

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    Vielen Dank für Ihre Nachrichten.

    Leider sind die anti-human IgGs nicht die Kontrollen, die wir Ihnen als Isotypkontrolle für die IL9- und IL9R- Antikörper empfehlen würden. (Damit würden Sie sicher eine Färbung erh...

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    Hi there,

    Thanks for getting back to me with all the info - that is great to know. I am going to be uisng a secondary antibody which I have purchased from Dako and used in previous IHC experiments where I used rabbit polyclonal and mouse monocl...

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    Thank you for getting back to me.

    I have seen the correspondence you have had with my colleague Claudia over the ab4360 slide, she wrote the following:

    These slides do not automatically contain this region (syncytiotrophoblast area), w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"