Overview

  • Product nameRabbit polyclonal IgGSee all Rabbit isotype controls ...
  • Specificityab27472 does not react with any antigen of human or animal cells.
  • Tested applicationsIHC-P, IP, Sandwich ELISAmore details
  • Species reactivity
    ab27472 does not react with any species.
  • General notesThe concentration of this prediluted antibody is 2 µg/ml.

Properties

  • FormPrediluted
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab27472 is an affinity purified IgG. It was purified from serum collected from a rabbit prior to immunization.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas
  • Alternative names
    • rabbit isotype control

Applications

Our Abpromise guarantee covers the use of ab27472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. Use neat for 10 minutes at room temperature. Pretreatment: same as primary antibody.
IP Use at an assay dependent dilution. PubMed: 21088000
Sandwich ELISA Use at an assay dependent dilution. PubMed: 19243500

References for Rabbit polyclonal IgG (ab27472)

This product has been referenced in:
  • Dawson MA  et al. Recurrent mutations, including NPM1c, activate a BRD4-dependent core transcriptional program in acute myeloid leukemia. Leukemia 28:311-20 (2014). Read more (PubMed: 24220271) »
  • Aza-Carmona M  et al. NPPB and ACAN, two novel SHOX2 transcription targets implicated in skeletal development. PLoS One 9:e83104 (2014). Read more (PubMed: 24421874) »

See all 11 Publications for this product

Product Wall

ab27472's concentration is indeed around 2 ng/ml depending on the lot. This is because this antibody is already prediluted for customers to use. If you wish to purchase the non-diluted form of rabbit polyclonal IgG, please consider ab27478 which is sol...

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Thank you for contacting us.

The price for Ireland is €360 + €38 delivery charges.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


I think ab27472 that you have selected is a great isotype control for ab6672 as it is a rabbit polyclonal IgG and ab6672 is also a rabbit polyclonal with isotype IgG.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any anti-CRM1 antibody tested in the species and application you are using...

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Thank you for your enquiry. I wish to sincerely apologize for the delay.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets....

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Thank you for your reply.
Below are the 2 products that I would recommend for you:
Isotype control - http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
Secondary - http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H...

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Thank you for contacting Abcam.
I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommen...

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Thank you for contacting us.

The concentrated form of ab27472 is ab27478.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an...

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Vielen Dank für Ihre Anfrage.

Für ab111915 wurden 20 µg Lysat aufgetragen, für ab66106 waren es10 µg (siehe Datenblatt). Normalerweise sind - je nach Expressionslevel in der Positivkontrolle - 20 bis maximal 50 &mic...

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Vielen Dank für Ihre Nachrichten.

Leider sind die anti-human IgGs nicht die Kontrollen, die wir Ihnen als Isotypkontrolle für die IL9- und IL9R- Antikörper empfehlen würden. (Damit würden Sie sicher eine Färbung ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"