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Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)

Submit an Abreview Q&A (2)Specific References (12)

Overview

  • Product nameRabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP)See all IgY secondary antibodies ...
  • Target speciesChicken
  • Tested applicationsICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB more details
  • Immunogen

    chicken IgY whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate (Do not add Sodium Azide!)
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesHorseradish Peroxidase (HRP).
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/1000 - 1/5000.
Dot Blot Dot: Use at an assay dependent concentration.
ELISA ELISA: 1/145000.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.
Immunomicroscopy IM: Use at an assay dependent concentration.
WB WB: 1/2500 - 1/10000.

Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) images

  • Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Ab6753 was used at dilution 1/2000 with the primary antibody ab16293 in IHC-Fr. See the review on ab16293.
  • ELISA - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    ELISA - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Ab6753 was used at dilution 1/10000 with the primary antibody ab15879 in ELISA. See the review on ab15879.
  • Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Ab6753 was used at dilution 1/5000 with the primary antibody ab19130 in WB. See the review on ab19130.

References for Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)

This product has been referenced in:
  • Vergnes L  et al. Heart-type Fatty Acid-binding Protein Is Essential for Efficient Brown Adipose Tissue Fatty Acid Oxidation and Cold Tolerance. J Biol Chem 286:380-90 (2011). IHC-Fr . Read more (PubMed: 21044951) »
  • Koshimizu E  et al. Embryonic senescence and laminopathies in a progeroid zebrafish model. PLoS One 6:e17688 (2011). WB . Read more (PubMed: 21479207) »

See all 12 Publications for this product

Publishing research using ab6753 ? Please let us know so that we can cite the reference in this datasheet

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Phoned because the customer is seeing very high background on the blot everywhere but the lanes which contain sample. Still getting good, clean signal on sample but was curious about this response.

Thank you for contacting us.

I have had the opportunity to speak with my collegues regarding this issue.We have seen similar patterns when the gel is over-heating during transfer or contaminants are present in the transfer buffer or equipment...

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Recently, I bought 5 antibodies from abcam, which are: 1 x GABARAPL2 antibody(ab15858) 1 x CREB antibody (ab5803) 1 x Leptin Receptor antibody (ab7211) 1 x ...

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Le pido que nos disculpe por la falta en su orden. He hecho una nueva orden cual le sera enviada lo mas pronto posible. Porfavor refiera al numero de orden 68450 si necesita mas información sobre el estado de su orden. Otra vez, porfavor disculpe la fal...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"