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AbExcel

Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)

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Overview

  • Product nameRabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP)See all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WB more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notesMolar enzyme: antibody protein ratio is 1:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/100 - 1/1000.
ELISA ELISA: 1/5000 - 1/50000. (Primary).
WB WB: 1/5000 - 1/50000. (Chemiluminescent).

Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) images

  • Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)
    Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)
    All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane. 

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/20000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097)

ab97097 has not yet been referenced specifically in any publications.

Publishing research using ab97097 ? Please let us know so that we can cite the reference in this datasheet

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I would like to order a complete set of antibodies and controls from Abcam for WB analysis. Could you check if these antibodies would suit for the analysis. In addition, could you recommend some positive contro...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"