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Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP), pre-adsorbed (ab7133)

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Overview

  • Product nameRabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesGoat
  • Tested applicationsDot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF more details
  • Minimal
    cross-reactivity
    Human more details
  • Immunogen

    Goat IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C. Do not freeze.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot Dot: Use at an assay dependent concentration.
ELISA ELISA: 1/10000.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.
Immunomicroscopy IM: Use at an assay dependent concentration.
WB WB: Use at an assay dependent dilution.
ICC/IF ICC/IF: 1/200 - 1/1000.

References for Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP), pre-adsorbed (ab7133)

ab7133 has not yet been referenced specifically in any publications.

Publishing research using ab7133 ? Please let us know so that we can cite the reference in this datasheet

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Rb anti-goat(H+L)-AP, Cat No ab7133-100. The problem being the conjugate cross-reacts with human proteins, although it is supposed to be cross absorbed against human/mouse.My assays are western blots against human pbmc lysates.The controls I set up were...

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Thank you for your enquiry. 1. How much lysate was loaded? We would suggest 5uL for 10x8cm gel. 2. Try to titer up the secondary antibody. The problem seems like a non-specific binding caused by too much antigen and/or antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"