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AbExcel

Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100)

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Overview

  • Product nameRabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WB more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/200 - 1/5000.
ELISA ELISA: 1/10000 - 1/100000. (Primary).
WB WB: 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) images

  • Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100)
    Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100)
    All lanes :
    Anti-beta Actin antibody (ab8229) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane. 
    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/10000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References for Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100)

ab97100 has not yet been referenced specifically in any publications.

Publishing research using ab97100 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab97100 Submit a Question for ab97100

Displaying 1 - 5 of 5 results for Abreviews and Q&A

10
Votes: Highest First

I was not getting any staining in IHC-P using this ab13611, and Envision dual link system HRP secondary antibody. Thanks to your ab97100 free sample, I am now seeing a slight staining pattern at 1:1000 dilution.

Thank you for trying our suggestion of using ab97100 instead of the staining kit. You may want to try increasing the dilution to 1:200 to get stronger staining.

A user has a problem with "Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)": Antibody solution includes precipitates colored brown.
Even she centrifuged it to eliminate these precipitates as mentioned on the datashe...

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Thank you for contacting us.

ab97100; one freeze thaw cycle should not be having any effect on quality so please recommend customer using the antibody as they would like to.

ab6721; please centrifuge out the brown precipitates. You may h...

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Product code: 111976
Lot number: GR62379-1

Inquiry: Dear Abcam Scientific Support, I'm finding some problems using your primary antibody ab-111976 anti-PAR1.
My protocol is:
8.5% acrylamide gel, 90 minutes of transfer on nitrocellulos...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.
...

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What is best secondary antibody you can offer for ab10256.

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Ple...

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Hi,   I just would like to know what are the developer kits for the following references: ·         ab97100. ·         ab97107.  

Thank you for contacting us with your question. For the alkaline phosphatase conjugated antibody ab97107, you can use the liquid fast-red kit ab64254- http://www.abcam.com/Liquid-Fast-Red-Substrate-Kit-ab64254.html For the HRP conjugated antibody...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"