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Rabbit anti-Mouse IgG H&L (Biotin) secondary antibody (ab97044)

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Overview

  • Product nameRabbit anti-Mouse IgG H&L (Biotin) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsELISA, ICC/IF, IHC-P, WB more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notesBiotin/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/20000 - 1/400000. (Primary).
ICC/IF ICC/IF: 1/200 - 1/1000.
IHC-P IHC-P: 1/500 - 1/5000.
WB WB: 1/20000 - 1/400000. (Chemiluminescent).

References for Rabbit anti-Mouse IgG H&L (Biotin) secondary antibody (ab97044)

ab97044 has not yet been referenced specifically in any publications.

Publishing research using ab97044 ? Please let us know so that we can cite the reference in this datasheet

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I have got a question about second antibodies used for Western Blot. At the moment I am using Polyclonal Rabbit-anti-mouse immunoglobulins/HRP as second antibody. We would like to add a step for strengthening the signal, like using Biotin. Is it possible ...

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Thank you for contacting us.

While it is theoretically possible to amplify your signal by using a biotinylated secondary antibody with an anti-biotin tertiary antibody, it is not commonly done. Because streptavidin binds to biotin with a very hi...

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Hello, I just wanted to confirm that this antibody has the ability to recognize both "mouse and rabbit" regions and can be used as secondary antibody for recognizing Mouse Antibodies and Rabbit Antibodies. Also is there any technical specifications as com...

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Thank you for your inquiry. ab64257 is indeed suitable for the detection of Mouse and Rabbit IgG. It can be used as a secondary antibody when used with rabbit or mouse primary antibodies. About ab64256 and ab97044, all of the information we have fo...

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Customer kindly requested Biotin conjugated secondary antibodies for ab2378, ab5690, ab103573, ab10558, ab955, ab53444, ab9018

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"