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Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed (ab98784)

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Overview

  • Product nameRabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesMouse
  • Tested applicationsIHC-P, ICC/IF, ELISA, WB more details
  • Minimal
    cross-reactivity
    Human, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/500 - 1/5000.
ICC/IF ICC/IF: 1/200 - 1/1000.
ELISA ELISA: 1/20000 - 1/400000. as primary
WB WB: 1/20000 - 1/400000. chemiluminescent detection

Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed (ab98784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed (ab98784)This image is courtesy of an anonymous Abreview
    Immunohistochemical analysis of dog skin tissue, detecting staining of NQO1 with ab98784.

    Tissue was fixed with formaldehyde and blocked with none for ; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (ab28947 Mouse monoclonal [A180] to NQO1 at 1/175 in BSA in TBS) for 45 minutes. ab98784 (1/500) was used as the secondary antibody.

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References for Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed (ab98784)

ab98784 has not yet been referenced specifically in any publications.

Publishing research using ab98784 ? Please let us know so that we can cite the reference in this datasheet

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Thank you very much for your reply.
I did vary goat anti-mouse IgG concentration, 1:25, 1:50, 1:100. In
all cases, even without primary antibody, I do see signals in
controls. Here are the conditions used:
goat anti-mouse IgG: var...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

Since the no primary control is showing staining, it's probably an issue with the secondary antibody...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"