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Rabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibody (ab8517)

Submit an Abreview Q&A (1)Specific References (4)

Overview

  • Product nameRabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibodySee all IgG + IgM + IgA secondary antibodies ...
  • Description
    Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC)
  • Target speciesMouse
  • SpecificityAntibodies were raised in rabbit to mouse IgG, IgM and IgA
  • Tested applicationsICC/IF, IHC-P more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality Polyclonal
  • General notesF/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent dilution. PubMed: 15483223
IHC-P IHC-P: Use at an assay dependent dilution.

Rabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibody images

  • Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC) (ab8517)
    Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Mouse IgG+IgM+IgA (FITC) (ab8517)
    Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.

References for Rabbit anti-Mouse IgG + IgM + IgA (FITC) secondary antibody (ab8517)

This product has been referenced in:

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compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"