Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab102169)

Overview

• Product nameRabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP)See all IgG secondary antibodies ...
• Target speciesRat
• SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
• Tested applicationsIHC-P, ICC, ELISA, WB more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Rat
  • IgG
  • Enzyme
  • Alkaline Phos

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications

Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) images

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  Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab102169)

  All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane. Secondary Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/2000 dilution Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/10000 dilution Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes Abcam recommends blocking with 3% milk to reproduce this western blot data.