Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)
Overview
- Product nameRabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP)See all IgG secondary antibodies ...
- Target speciesRat
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Tested applicationsIHC-P, ICC, ELISA, WB more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
- Clonality Polyclonal
- IsotypeIgG
- General notesPart of the AbExcel range.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab102172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/200 - 1/5000. |
| ICC | ICC: 1/200 - 1/5000. |
| ELISA | ELISA: 1/10000 - 1/100000. (as primary) |
| WB | WB: 1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000). |
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) images
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Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml
Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 52 kDaAdditional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 secondsAbcam recommends blocking with 3% milk to reproduce this western blot data.
References for Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172)
ab102172 has not yet been referenced specifically in any publications.
