Anti-Rac1 antibody [0.T.127] (ab33186)

45Four stars 13 Abreviews

6 Specific references

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Publishing research using ab33186? Please let us know so that we can cite the reference in this datasheet

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ab33186 has been referenced in 6 publications.

Chatterjee S et al. Peroxiredoxin 6 Phosphorylation and Subsequent Phospholipase A2 Activity Are Required for Agonist-mediated Activation of NADPH Oxidase in Mouse Pulmonary Microvascular Endothelium and Alveolar Macrophages. J Biol Chem 286:11696-706 (2011). WB; Mouse. PubMed: 21262967
Kim JY et al. The RhoG/ELMO1/Dock180 Signaling Module Is Required for Spine Morphogenesis in Hippocampal Neurons. J Biol Chem 286:37615-24 (2011). WB; Rat. PubMed: 21900250
Yang W et al. Up-regulation of Tiam1 and Rac1 correlates with poor prognosis in hepatocellular carcinoma. Jpn J Clin Oncol 40:1053-9 (2010). IHC-P; Human. PubMed: 20522449
Zanca C et al. PED interacts with Rac1 and regulates cell migration/invasion processes in human non-small cell lung cancer cells. J Cell Physiol 225:63-72 (2010). WB; Human. PubMed: 20648624
Qi Y et al. Prognostic value of Tiam1 and Rac1 overexpression in nasopharyngeal carcinoma. ORL J Otorhinolaryngol Relat Spec 71:163-71 (2009). Human. PubMed: 19506399
Smith TK et al. Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity. Proc Natl Acad Sci U S A 105:8298-303 (2008). WB; Mouse. PubMed: 18541910

Publishing research using ab33186? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Western blot - Rac1 antibody [0.T.127] (ab33186)

Anti-Rac1 antibody [0.T.127] (ab33186) at 1/750 dilution (for 14 hours at 4°C) + Mouse brain whole tissue lysate at 15 µg

Secondary
An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 21 kDa
Observed band size : 21 kDa

Exposure time : 2 minutes

Blocking Step: 5% Milk for 1 hour at 22°C

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody [0.T.127] (ab33186)

ab33186 staining Rac1 in Mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA 1 hour at 20ºC ; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/750) for 4 hours at 20ºC. An HRP-conjugated Goat anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview