Anti-Rac1 antibody [0.T.127] (ab33186)

Overview

• Product nameAnti-Rac1 antibody [0.T.127]See all Rac1 primary antibodies ...
• Description
  Mouse monoclonal [0.T.127] to Rac1
• SpecificityRecognizes Rac at 21kD.Slight Rac 2 cross-reactivity
• Tested applicationsIHC-Fr, WB, Flow Cyt, IHC (PFA fixed), ICC/IF, ELISA, IP, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Human
  
• Immunogen

  Recombinant protein containing the full length human Rac.

• Positive controlWB/IP: rat brain microsomal protein preparation IHC: rat brain sections fixed first with PBS, 4% paraformaldehyde, 2% acetic acid for 30 minutes followed by ethanol:acetic acid [95:5] for 2 minutes. Affinity Precipitation Assay: lysates of 3T3 fibroblasts

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 40% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number0.T.127
• IsotypeIgG2b
• Research Areas
  • Cancer
  • Signal transduction
  • G protein signaling
  • Small G proteins
  • Ras family

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • Small G Proteins
  • Ras Family

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionPlasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
  Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
• Tissue specificityIsoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
• Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
• DomainThe effector region mediates interaction with DEF6.
• Post-translational
  modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
• Cellular localizationCell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P63000 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5879 Human
  • Entrez Gene: 19353 Mouse
  • Entrez Gene: 363875 Rat
  • Omim: 602048 Human
  • SwissProt: P63000 Human
  • SwissProt: P63001 Mouse
  • SwissProt: Q6RUV5 Rat
  • Unigene: 413812 Human

  • Unigene: 292510 Mouse
  • Unigene: 342177 Mouse
  • Unigene: 29157 Rat

  see all

• Alternative names
  Cell migration inducing gene 5 protein antibodyCell migration-inducing gene 5 protein antibodyMGC111543 antibody

  MIG 5 antibodyMIG5 antibodyMigration inducing gene 5 antibodyMigration inducing protein 5 antibodyp21 Rac1 antibodyp21-Rac1 antibodyRac 1 antibodyRAC1 antibodyRAC1_HUMAN antibodyRas like protein TC25 antibodyRas-like protein TC25 antibodyRas-related C3 botulinum toxin substrate 1 antibodyrho family small GTP binding protein Rac1 antibodyTC 25 antibodyTC25 antibody

  see all

Anti-Rac1 antibody [0.T.127] images

• 

  Western blot - Rac1 antibody [0.T.127] (ab33186)
  Anti-Rac1 antibody [0.T.127] (ab33186) at 1/750 dilution (for 14 hours at 4°C) + Mouse brain whole tissue lysate at 15 µg
  
  Secondary
  An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 21 kDa
  Observed band size : 21 kDa
  
  
  Exposure time : 2 minutes

  This image is courtesy of an anonymous Abreview

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody [0.T.127] (ab33186)This image is courtesy of an anonymous Abreview
  ab33186 staining Rac1 in Mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA 1 hour at 20°C ; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. An HRP-conjugated Goat anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.

  See Abreview